大蒜素通过抑制OPN表达和NFκB通路活化对大鼠肾结石形成的作用研究  

Study on the Effect of Allicin on Rat Kidney Stone Formation by Inhibiting OPN Expression and Activation of NF‑κB Pathway

作　　者：徐旭红[1] 孙建明[1] 张伦中[1] 许良余[1] 曾向阳[1] 孙龙飞[1] 陈晓峰[1] XU Xuhong;SUN Jianming;ZHANG Lunzhong;XU Liangyu;ZENG Xiangyang;SUN Longfei;CHEN Xiaofeng(Department of Urology,the First People's Hospital of Chenzhou City,Hunan Chenzhou 423000,China)

机构地区：[1]郴州市第一人民医院泌尿外科,湖南郴州423000

出　　处：《生物技术进展》2022年第2期305-312,共8页Current Biotechnology

摘　　要：大蒜素可改善草酸诱导的肾小管上皮细胞损伤,以肾结石大鼠为研究对象,探讨大蒜素对肾结石大鼠的作用及其可能的机制。采用1%乙二醇+2%氯化铵混合液灌胃造模(空白组除外),分别灌胃大蒜素7.5 mg·kg^(-1)(低剂量大蒜素组)、15 mg·kg^(-1)(高剂量大蒜素组)、胃枸橼酸氢钾钠颗粒0.6 g·kg^(-1)(阳性对照组),其余组灌胃0.9%氯化钠溶液(空白组),检测各组大鼠与肾结石疾病相关的指标。与空白组相比,模型组大鼠肾指数、肌酐(creatinine,Cr)、血清尿素氮(bloodureanitrogen,BUN)水平和天冬氨酸转氨酶(aspartateaminotransferase,AST)、谷丙转氨酶(alanineaminotransferase,ALT)活性及24 h尿量、尿液中草酸、钙和磷含量显著升高(P<0.05),草酸钙结晶评分显著升高(P<0.05),镁含量显著降低(P<0.05),骨桥蛋白(osteopontin,OPN)表达显著升高(P<0.05),核因子κB(nuclearfactor‑κB,NF‑κB)通路活化;与模型组相比,低剂量大蒜素组、高剂量大蒜素组和阳性对照组大鼠肾指数、Cr、BUN水平和AST、ALT活性、24 h尿量、尿液中草酸、钙和磷含量显著降低(P<0.05),草酸钙结晶评分显著降低(P<0.05),镁含量显著升高(P<0.05),OPN表达显著降低(P<0.05),NF‑κB通路被抑制。结果表明,大蒜素通过改善大鼠肾功能指标、抑制骨桥蛋白表达和NF‑κB通路活化进而抑制肾结石形成。Allicin can improve the injury of renal tubular epithelial cells induced by oxalic acid,this study took rats with kidney stones as the research object to explore the effect of allicin on rats with kidney stones and its possible mechanism.1%ethylene glycol+2%ammonium chloride method to prepare a model of rats with kidney stones(except blank group).Allicin 7.5 mg·kg^(-1)(low allicin dose group),15 mg·kg^(-1)(high allicin dose group),potassium sodium hydrogen citrate 0.6 g·kg^(-1)(positive control group),and 0.9%sodium chloride solution for other groups(blank group)were gavaged respectively.The indexes related to kidney stone disease in each group were detected.Compared with the blank group,the model group had significantly higher renal index,Cr,BUN levels,aspartate aminotransferase(AST),alanine aminotransferase(ALT)activity,24 h urine output,urine oxalic acid,calcium and phosphorus content(P<0.05),and the calcium oxalate crystallization score was significantly increased(P<0.05),magnesium content was significantly decreased(P<0.05),osteopontin(OPN)expression was significantly increased(P<0.05),nuclear factor‑κB(NF‑κB)pathway was activated.Compared with the model group,the renal index,Cr,BUN levels and AST,ALT activity,24 h urine output,urine oxalic acid,calcium and phosphorus content in the low allicin dose group,high dose group and the positive control group were significantly reduced(P<0.05),and calcium oxalate crystal score was significantly reduced(P<0.05),the magnesium content was significantly increased(P<0.05),OPN expression was significantly reduced(P<0.05),and the NF‑κB pathway was inhibited.Allicin can inhibit the formation of kidney stones by improving the renal function indexes of rats,inhibiting the expression of osteopontin and the activation of NF‑κB pathway.

关 键 词：大蒜素 肾结石 骨桥蛋白 NF‑κB通路 

分 类 号：R692.4[医药卫生—泌尿科学]