基于二维相关红外光谱的陈皮快速鉴别研究  

Rapid identification of citri reticulatae pericarpium based on two-dimensional correlation infrared spectroscopy

作　　者：杨济齐 沈婉莹 魏晓芳 杜文超 张景照[2,3] 唐旭东 YANG Ji-qi;SHEN Wan-ying;WEI Xiao-fang;DU Wen-chao;ZHANG Jing-zhao;TANG Xu-dong(Gansu University of Chinese Medicine,Lanzhou 730000;Key Laboratory of Innovative Traditional Chinese Medicine and Natural Medicine,Research Institute of Tsinghua University in Shenzhen,Shenzhen Guangdong 518000;Guangdong Innovation Traditional Chinese Medicine and Natural Medicine Research Engineering Center,Shenzhen Guangdong 518000)

机构地区：[1]甘肃中医药大学,兰州730000 [2]深圳清华大学研究院创新中药及天然药物研究重点实验室,广东深圳518000 [3]广东省创新中药及天然药物研究工程中心,广东深圳518000

出　　处：《中南药学》2022年第3期544-550,共7页Central South Pharmacy

基　　金：深圳市科技计划项目基础研究(学科布局)(No.JCYJ20160301100720906;No.JCYJ20160510141910129)。

摘　　要：目的对比不同产区、不同储存年限陈皮水分及橙皮苷、川陈皮素、橘皮素含量的差异,建立不同产区、不同储存年限陈皮的二维相关红外光谱分析方法。方法采用甲苯法对14批陈皮的水分进行测定,高效液相色谱法测定橙皮苷、川陈皮素、橘皮素含量。对不同产区、不同储存年限的14批陈皮样品进行二维相关红外光谱分析。结果不同产区、不同储存年限陈皮水分及橙皮苷、川陈皮素、橘皮素含量有所不同。二维相关红外光谱显示,不同产区、不同储存年限陈皮的相关峰强度、位置有明显差异。结论二维相关红外光谱可以为陈皮的品质评价、鉴别、质量控制提供依据,是一种快速有效的方法。Objective To compare the content of moisture,hesperidin,nobiletin and tangeretin in citri reticulatae pericarpium from different production areas with different storage years,and to establish a two-dimensional correlation infrared spectroscopy analysis method.Methods The moisture content of 14 batches of citri reticulatae pericarpium were determined by toluene method.The content of hesperidin,nobiletin and tangeretin were determined by HPLC.The 14 batches of citri reticulatae pericarpium were analyzed by two-dimensional correlation infrared spectroscopy.Results The content of moisture,hesperidin,nobiletin and tangeretin of different production areas and storage years of citri reticulatae pericarpium samples was different.There were obvious differences in the relevant peak intensity and position of citri reticulatae pericarpium from different production areas with different storage years in the two-dimensional correlation infrared spectra.Conclusion Two-dimensional correlated infrared spectroscopy can provide evidence for the evaluation,identification and quality control of citri reticulatae pericarpium,which is rapid and effective.

关 键 词：陈皮 二维相关红外光谱 傅里叶变换红外光谱 产区 储存年限 水分 含量测定 

分 类 号：R927[医药卫生—药学]