灭多威对斑马鱼胚胎抗氧化防御系统的影响  

Effects of methomyl on antioxidant defense system of zebrafish embryos

作　　者：陆妍 陈曦 裘丽萍[2] 胡庚东[1,2] 宋超[1,2] 范立民[1,2] 郑尧[1,2] 孟顺龙[1,2] 陈家长[1,2] LU Yan;CHEN Xi;QIU Liping;HU Gengdong;SONG Chao;FAN Limin;ZHENG Yao;MENG Shunlong;CHEN Jiazhang(Wuxi Fishery College,Nanjing Agricultural University,Wuxi 214081,China;Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences/Laboratory of Quality&Safety Risk Assessment for Aquatic Products on Environmental Factors(Wuxi),Ministry of Agriculture and Rural Affairs/Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries,Chinese Academy of Fishery Sciences,Wuxi 214081,China)

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,农业农村部水产品质量安全环境因子风险评估实验室(无锡),中国水产科学研究院内陆渔业生态环境和资源重点开放实验室,江苏无锡214081

出　　处：《农业环境科学学报》2022年第3期472-480,共9页Journal of Agro-Environment Science

基　　金：国家现代农业产业技术体系(CARS-46);国家重点研发计划项目(2020YFD0900502)。

摘　　要：为探究灭多威对斑马鱼胚胎抗氧化防御系统的影响及氧化损伤效应,将受精后6 h内的斑马鱼胚胎暴露在浓度为0、2、20、200μg·L;的灭多威溶液中,分别在24、48、72、96 h时测定斑马鱼体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)、还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)的活性及丙二醛(MDA)的含量。暴露于灭多威后,SOD活性呈现先降低后升高再降低的趋势,各浓度处理组与对照组差异显著(P<0.05);CAT活性表现为先被诱导后抑制减弱的效应,其中20、200μg·L;处理组CAT活性显著高于对照组(P<0.05);GR、GSH、GSSG活性呈现先升高后降低的趋势;与对照组相比,各浓度处理组斑马鱼胚胎体内MDA含量增加,呈先增加后减少再增加的趋势。灭多威对斑马鱼胚胎具氧化应激效应,造成鱼体内抗氧化防御因子活性水平及MDA含量发生改变,干扰抗氧化防御系统正常工作,具有氧化损伤效应。To explore the effects of methomyl on the antioxidant defense system and oxidative damage in zebrafish embryo development,zebrafish embryos were exposed to methomyl solutions of 0, 2, 20 μg·L;, and 200 μg·L;concentrations within 6 h after fertilization. The contents and activities of superoxide dismutase(SOD), catalase(CAT), glutathione reductase(GR), reduced glutathione(GSH), oxidized glutathione(GSSG), and malondialdehyde(MDA)were measured at 24, 48, 72 h, and 96 h, respectively. After exposure to methomyl, the SOD activity decreased at first, then increased and decreased, with significant differences between each concentration group and the control group(P<0.05). The CAT activity was first induced and then inhibited;the CAT activity in 20 μg·L;and 200 μg·L;treatment groups was significantly higher than that in the control group(P<0.05). The GR, GSH, and GSSG activity increased at first and then decreased.Compared with the control group, the MDA content in zebrafish embryos in each concentration group increased, first increasing, then decreasing, and finally increasing again. Methomyl has an oxidative stress effect on zebrafish embryos, changing the activity level of antioxidant defense factors and MDA content in zebrafish embryos by inhibiting or promoting it, which interferes with the normal operation of the antioxidant defense system, with oxidative damage effects.

关 键 词：灭多威 斑马鱼 胚胎 抗氧化防御因子 氧化损伤 

分 类 号：X171.5[环境科学与工程—环境科学]