超声-负压法提取藜麦皂苷及其活性研究  

Study on extraction of quinoa saponins by ultrasonic negative pressure method and its activity

作　　者：芦晓芳[1] 唐雨薇 高春艳[1] 张永坡[1] 岳爱琴[1] 赵晋忠[1] LU Xiao-fang;TANG Yu-wei;GAO Chun-yan;ZHANG Yong-po;YUE Ai-qin;ZHAO Jin-zhong(Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学,太谷030801

出　　处：《天然产物研究与开发》2022年第3期465-472,共8页Natural Product Research and Development

基　　金：山西省201省合署改革博科研启动(J242098140)。

摘　　要：本文以藜麦皂苷为研究对象,基于单因素试验,以皂苷提取率为指标,通过响应面法优化,结合藜麦粉末SEM表征,以获得皂苷的超声-负压提取最佳工艺参数。藜麦皂苷粗提物经正丁醇萃取后,探讨其体外抗氧化活性及相关机理。实验结果表明:超声-负压法提取藜麦皂苷最佳工艺参数:超声时间20 min、超声温度59℃、负压0.064 MPa,该条件下皂苷得率(预测值12.37 mg/g,实验值11.95±0.034 mg/g)最高,三因素对皂苷得率的影响顺序:负压>超声温度>超声时间。藜麦皂苷在浓度范围(0~0.25 mg/mL)内有较强的还原力和清除DPPH自由基的能力,抗氧活化活性与其浓度呈正相关。In this paper,quinoa saponins as the research object,based on single factor experiment,with saponins extraction yield as index,through response surface method optimization,combined with quinoa powder SEM characterization,in order to obtain the optimal process parameters of ultrasonic-vacuum extraction of saponins.After the crude extract of quinoa saponins was extracted with n-butanol,its antioxidant activity and related mechanism were investigated.The experimental results showed that the optimal process parameters for the extraction of quinoa saponins by ultrasonic-negative pressure method were as follows∶ultrasonic time 20 min,ultrasonic temperature 59℃,negative pressure 0.064 MPa.Under this condition,extraction yield was the highest(the predicting value was 12.37 mg/g,and the experimental value was 11.95±0.034 mg/g).The influence order of the three factors on the yield of quinoa saponins was as follows∶negative pressure>ultrasonic temperature>ultrasonic time.Quinoa saponins had strong reducing power and DPPH free radical scavenging ability in the concentration range(0-0.25 mg/mL),and its antioxidant activity was positively correlated with its concentration.

关 键 词：藜麦皂苷 超声-负压辅助提取 响应面优化 抗氧化活性 

分 类 号：Q69[生物学—生物物理学]