Molecular cloning and functional characterization of an isoflavone glucosyltransferase from Pueraria thomsonii  

作　　者：DUAN Hai-Yan WANG Jian ZHA Liang-Ping PENG Hua-Sheng ZHAO Yu-Ping YUAN Yuan HUANG Lu-Qi 

机构地区：[1]School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China [2]State Key Laboratory Breeding Base of Dao-di Herbs,China Academy of Chinese Medical Sciences,Beijing 100700,China [3]Research Unit of DAO-DI Herbs,Chinese Academy of Medical Sciences,Beijing 100700,China

出　　处：《Chinese Journal of Natural Medicines》2022年第2期133-138,共6页中国天然药物（英文版）

基　　金：supported by the National Key Research and Development Program of China(Nos.2017YFC1702901 and 2017YFC1701601);CAMS Innovation Fund for Medical Sciences(No.2019-I2M-5-065)。

摘　　要：Pueraria thomsonii has long been used in traditional Chinese medicine.Isoflavonoids are the principle pharmacologically active components,which are primarily observed as glycosyl-conjugates and accumulate in P.thomsonii roots.However,the molecular mechanisms underlying the glycosylation processes in(iso)flavonoid biosynthesis have not been thoroughly elucidated.In the current study,an O-glucosyltransferase(PtUGT8)was identified in the medicinal plant P.thomsonii from RNA-seq database.Biochemical assays of the recombinant PtUGT8 showed that it was able to glycosylate chalcone(isoliquiritigenin)at the 4-OH position and glycosylate isoflavones(daidzein,formononetin,and genistein)at the 7-OH or 4′-OH position,exhibiting no enzyme activity to flavonones(liquiritigenin and narigenin)in vitro.The identification of PtUGT8 may provide a useful enzyme catalyst for efficient biotransformation of isoflavones and other natural products for food or pharmacological applications.

关 键 词：Flavonone ISOFLAVONE GLUCOSYLTRANSFERASE 4′-O-glucosylation 7-O-glucosylation 

分 类 号：Q819[生物学—生物工程]