黄连对2型糖尿病大鼠调节性T细胞及相关炎症因子的影响  

Effect of rhizoma coptidis on regulatory T cells and related inflammatory factors in type 2 diabetic rats

作　　者：郭志利[1] 左晓琦 张梅[3] 康秉南 姚玉英[1] GUO Zhili;ZUO Xiaoqi;ZHANG Mei;KANG Bingnan;YAO Yuying(Shijiazhuang Medical College, Shijiazhuang 050599, Hebei, China;The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, China;Shijiazhuang Vocational College of Technology and Information, Shijiazhuang 050091, Hebei, China)

机构地区：[1]石家庄医学高等专科学校,河北石家庄050599 [2]河北医科大学第二医院,河北石家庄050000 [3]石家庄科技信息职业学院,河北石家庄050091

出　　处：《现代中西医结合杂志》2022年第5期619-623,717,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省中医药管理局科研计划项目(2019501)。

摘　　要：目的探讨黄连对2型糖尿病大鼠调节性T细胞及相关炎症因子的影响。方法以高脂高糖饲料联合链脲佐菌素诱导SD大鼠复制2型糖尿病模型,将造模成功的75只大鼠随机分为模型组、小檗碱低剂量组、小檗碱高剂量组各25只,另取25只正常SD大鼠作为正常组。造模后,小檗碱低、高剂量组分别给予0.125 mg/g和0.375 mg/g的小檗碱灌胃,正常组和模型组给予等体积的生理盐水灌胃。分别于灌胃2,4,6,8,12周后每组随机取5只大鼠,采集腹主动脉血,检测空腹血糖(FPG)和空腹胰岛素(FINS)水平;取大鼠脾脏组织,采用流式细胞仪检测CD4^(+)CD25^(+)调节性T细胞占比;Real-time PCR法检测脾脏组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、干扰素-γ(IFN-γ)mRNA表达量。结果与同时间段正常组比较,模型组大鼠灌胃2,4,6,8,12周后血清FPG水平及脾脏组织中TNF-α、IL-1β、IFN-γmRNA表达量均显著升高,血清FINS水平及脾脏组织中CD4^(+)CD25^(+)调节性T细胞占比均显著降低,差异均有统计学意义(P均<0.05);与同时间段模型组比较,小檗碱低、高剂量组大鼠灌胃2,4,6,8,12周后血清FPG水平及脾脏组织中TNF-α、IL-1β、IFN-γmRNA表达量均显著降低,血清FINS水平及脾脏组织中CD4^(+)CD25^(+)调节性T细胞占比均显著升高,差异均有统计学意义(P均<0.05);与同时间段小檗碱低剂量组比较,小檗碱高剂量组灌胃6,8,12周后血清FPG水平及脾脏组织中TNF-α、IL-1β、IFN-γmRNA表达量均更低,血清FINS水平及脾脏组织中CD4^(+)CD25^(+)调节性T细胞占比均更高,差异均有统计学意义(P均<0.05)。结论黄连可降低高脂饲料联合链脲佐菌素诱导的2型糖尿病大鼠血糖水平,上调血清胰岛素水平,其作用机制可能与调控调节性T细胞及炎症因子水平有关。Objective It is to investigate the effects of rhizoma coptidis on regulatory T cells and related inflammatory factors in type 2 diabetic rats.Methods The SD rats were induced to replicate the type 2 diabetes models with high-fat and high-sugar diet combined with streptozotocin,and 75 successful modeling rats were randomly divided into model group,low-dose berberine group,and high-dose berberine group,25 rats in each group,and another 25 normal SD rats were selected as the normal group.After modeling,the berberine low-dose and high-dose groups were respectively given 0.125 mg/g and 0.375 mg/g berberine by gavage,and the normal group and model group were given the same volume of normal saline by gavage.After 2,4,6,8,and 12 weeks of gavage,5 rats were randomly selected from each group,and their abdominal aortic blood was collected to detect the levels of fasting plasma glucose(FPG)and fasting insulin(FINS);their spleen tissue was taken to detect the proportion of CD4^(+)CD25^(+)regulatory T cells by flow cytometry,and to detect the expression of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interferon-γ(IFN-γ)mRNA by Real-time PCR method.Results Compared with the normal group at the same time period,the serum FPG level and the expression of TNF-α,IL-1βand IFN-γmRNA expressions in the spleen tissue of the rats were significantly increased,the serum FINS level and the proportion of CD4^(+)CD25^(+)regulatory T cells in the spleen tissue were significantly decreased in the model group after 2,4,6,8,and 12 weeks of gavage,and the differences were statistically significant(all P<0.05).Compared with the model group at the same time period,the serum FPG level and the expression of TNF-α,IL-1βand IFN-γmRNA expressions in the spleen tissue of the rats were significantly decreased,the serum FINS level and the proportion of CD4^(+)CD25^(+)regulatory T cells in the spleen tissue were significantly increased in the low dose and high dose groups of berberine after 2,4,6,8,and 12 weeks of gavage,and the differenc

关 键 词：小檗碱 2型糖尿病 调节性T细胞 炎症因子 

分 类 号：R-332[医药卫生]