干酪乳杆菌对便秘小鼠肠道菌群的影响  

Effect of Lactobacillus casei on intestinal flora in mice with constipation

作　　者：曾献春[1] 朱雅琴 王明明 李佳雯 刘蔓萩 庄伟伟[3] ZENG Xianchun;ZHU Yaqin;WANG Mingming;LI Jiawen;LIU Manqiu;ZHUANG Weiwei(College of Laboratory Medicine,Chengdu Medical College,Chengdu 610500,China;Xichong County,Agricultural and Rural Bureau,Nanchong 637200,China;Laboratory of Species Conservation and Regulation Biology of Special Environments in Xinjiang,International Research Center for Joint Control of Pests in Central Asia Region,Laboratory of Arid Zone Plant Adversity Biology,College of Life Sciences,Xinjiang Normal University,Urumqi 830054,China)

机构地区：[1]成都医学院检验医学院,四川成都610500 [2]西充县农业农村局,四川南充637200 [3]新疆特殊环境物种保护与调控生物学实验室,中亚区域有害生物联合控制国际研究中心,干旱区植物逆境生物学实验室,生命科学学院(新疆师范大学),新疆乌鲁木齐830054

出　　处：《食品与发酵工业》2022年第6期64-70,共7页Food and Fermentation Industries

基　　金：国家自然科学基金(30960047);四川省科技计划(2018JY0266)。

摘　　要：该试验探讨了从健康儿童肠道分离的干酪乳杆菌对便秘小鼠肠道粪便菌群组成的影响。选择体重为(20±2)g的小鼠72只,随机分为6组,分别为正常对照组、模型对照组、阳性药物对照组、干酪乳杆菌低剂量组、中剂量组和高剂量组。用复方地芬诺酯灌胃小鼠,建立便秘模型。正常对照组小鼠灌胃生理盐水,模型对照组小鼠灌胃复方地芬诺酯和生理盐水,阳性对照组灌胃复方地芬诺酯和嗜热链球菌,其余各组便秘小鼠灌胃复方地芬诺酯和相应浓度的干酪乳杆菌。灌胃3 d后,采集小鼠肠道内的粪便,用于分析菌群组成。结果表明,与正常对照组相比,便秘小鼠肠道菌群中乳杆菌属、另枝菌属丰度有不同程度的降低,而普雷沃氏菌属、norank_f_Muribaculaceae丰度升高。干酪乳杆菌干预3 d,优势菌群为norank_f_Muribaculaceae、乳杆菌属、拟普雷沃菌属、嗜冷杆菌属。与模型对照组相比,干酪乳杆菌组小鼠肠道菌群中普雷沃氏菌属、norank_f_Muribaculaceae丰度降低,另枝菌属丰度升高;干酪乳杆菌中剂量组、干酪乳杆菌高剂量组中乳杆菌属丰度升高。提示干酪乳杆菌可通过抑制条件致病菌普雷沃氏菌属的增殖,促进肠道中乳杆菌属、另枝菌属的生长,从而改善肠道菌群,达到缓解便秘的作用。The impact of different Lactobacillus casei doses on the intestinal microbiota in mice was evaluated in this experiment.Seventy-two mice weighing(20±2)g were selected and randomly divided into six groups:normal control group,model control group,positive drug control group,L.casei low-dose group,medium-dose group and high-dose group,respectively.Mice were fed with compound diphenoxylate to establish a constipation model.Mice in the normal control group were fed with saline,and mice in the model control group were fed with compound difenoxil and saline,meanwhile,mice in the positive control group were fed with compound difenoxil and Streptococcus thermophilus,and mice in the remaining groups with constipation were fed with compound difenoxil and corresponding concentrations of L.casei.After 3 days of gavage,feces were collected from the intestinal tract of the mice for analysis of flora composition.The results showed that the abundance of Lactobacillus and Alistipes in the intestinal flora of constipated mice was reduced to different degrees,while the abundance of Prevotella and norank_f_Muribaculaceae was increased compared with the normal control group.After 3 days of Lactobacillus casei intervention,the dominant flora were norank_f_Muribaculaceae,Lactobacillus,Prevotella and Cryobacillus.Compared with the model control group,the abundance of Prevotella and norank_f_Muribaculaceae in the intestinal flora of mice in L.casei groups decreased,and the abundance of Alistipes increased;the abundance of Lactobacillus in the L.casei medium-dose group and high-dose group increased.It is suggested that L.casei can improve the intestinal flora and relieve constipation by inhibiting the growth of Prevotella and promoting the growth of Lactobacillus and Alistipes in the intestinal tract.

关 键 词：干酪乳杆菌 肠道菌群 便秘 小鼠 高通量测序 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]