绵羊MYF6基因多态性对胴体瘦肉性状的影响  

Effects of MYF6 genetic polymorphism on carcass lean traits of sheep

作　　者：鲁玉洁 周辉通 柯娜 王继卿 黄兆春 梁维炜 甄慧敏 罗玉柱 刘秀 李少斌 LU Yujie;ZHOU Huitong;KE Na;WANG Jiqing;HUANG Zhaochun;LIANG Weiwei;ZHEN Huimin;LUO Yuzhu;LIU Xiu;LI Shaobin(Gansu Engineering Lab of Genetic Improvement in Ruminants,Gansu Key Laboratory of Herbivorous Animal Biotechnology,College of Animal Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070,China;Faculty of Agriculture and Life Sciences,Lincoln University,Lincoln 7647,New Zealand)

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃省草食动物生物技术重点实验室,甘肃省牛羊基因改良工程实验室,甘肃兰州730070 [2]新西兰林肯大学农业与生命科学学院,新西兰林肯7647

出　　处：《西北农林科技大学学报（自然科学版）》2022年第3期10-16,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：甘肃农业大学“伏羲青年英才培育计划”项目(Gaufx-02Y02);甘肃省基础研究创新群体项目(18JR3RA190);甘肃农业大学自列课题(GSAU-ZL-2015-033);甘肃省财政厅项目(033-041041)。

摘　　要：【目的】研究绵羊生肌因子6(myogenic factor 6,MYF6)基因的多态性,分析其对羔羊胴体瘦肉性状的影响。【方法】以215只新西兰罗姆尼公羔为对象,采集其血样,屠宰后测定其后腿、腰部和肩部瘦肉量等胴体指标。提取血样基因组DNA,采用PCR-SSCP检测MYF6基因5′UTR和第1外显子2个区域的多态性,分析核苷酸序列变异对羔羊胴体瘦肉性状的影响。采集绵羊乳腺、肝脏、心脏、背最长肌、脾脏、肺脏、卵巢和肾脏组织,以β-actin为内参基因,用RT-qPCR等方法检测MYF6基因的相对表达量。【结果】在第1外显子区域,检测到1个SNP(c.129 C>T),有A和B 2种等位基因,存在AA和AB 2种基因型。在5′UTR区域检测到1个3 bp的碱基缺失(c.-589~-590 del ATA),有C和D 2种等位基因,存在CC和DD 2种基因型。2个区域的基因型分布均处于Hardy-Weinberg平衡状态。MYF6基因第1外显子的基因型极显著影响了羔羊后腿、腰部和肩部3个部位的瘦肉量,以及总瘦肉量和腰部瘦肉比例(P<0.01)。MYF6基因在绵羊乳腺、肝脏、心脏等8个组织中均有表达,但以背最长肌中的表达量最高(P<0.05)。【结论】绵羊MYF6基因具有丰富的多态性,其基因型对胴体瘦肉性状有显著影响。【Objective】This study analyzed the genetic characteristics of myogenic factor(MYF6)gene in sheep and its effect on lean meat traits of lambs.【Method】A total of 215 New Zealand Romney males were investigated.After slaughter,the carcass indicators including lean yields from leg,loin and shoulder were measured.The blood samples were collected and the genomic DNA was extracted.Subsequently,the nucleotide sequence variations were detected in both exon 1 and 5′UTR regions of MYF6 using PCR-SSCP and the relative expression was compared between ovine tissues.【Result】Two variants(A and B)and one single nucleotide polymorphism(SNP)(c.129 C>T)were detected in exon 1,while two variants(C and D)and one 3-bp deletion(c.-589--590 del ATA)were found in the 5′UTR region.The genotype distributions in both exon 1 and 5′UTR followed Hardy-Weinberg equilibrium in these Romney males.The genotypes in exon 1 of MYF6 significantly affected lean yields in leg,loin and shoulder,total lean yield and the proportion of loin lean yield in these males(P<0.01).The RT-qPCR results found that MYF6 expressed in eight tissues with the highest in Longissimus dorsi muscle(P<0.05).【Conclusion】MYF6 gene had abundant polymorphism in sheep,and its genotype significantly affected lean meat traits of sheep carcass.

关 键 词：罗姆尼绵羊 MYF6基因 第1外显子 胴体性状 

分 类 号：S826[农业科学—畜牧学]