多异瓢虫不同发育阶段过冷却点及体液结冰点的测定  

Determination of supercooling point and freezing point of hymolymph in Hippodamia variegata(Coleoptera:Coccinellidae)at different developmental stages

作　　者：羿超群 谢佼昕 刘晓旭 唐浩宇 刘廷辉[1] 张永军[2] YI Chaoqun;XIE Jiaoxin;LIU Xiaoxu;TANG Haoyu;LIU Tinghui;ZHANG Yongjun(College of Plant Protection,Hebei Agricultural University,Baoding017001,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing100193,China;School of Ecology and Environment,Anhui Normal University,Wuhu241000,China)

机构地区：[1]河北农业大学植物保护学院,保定071000 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [3]安徽师范大学生态与环境学院,芜湖241000

出　　处：《植物保护》2022年第2期106-110,117,共6页Plant Protection

基　　金：国家自然科学基金(31972338)。

摘　　要：多异瓢虫Hippodamia variegata是新疆棉田优势捕食性天敌。为明确不同发育阶段的多异瓢虫的耐寒能力,本研究利用过冷却点仪对多异瓢虫的3龄、4龄幼虫,蛹,2日龄雌、雄成虫分别进行了过冷却点和体液结冰点的测定,并对其频次分布进行了分析。结果表明:蛹的过冷却点(-21.05±0.75)℃和体液结冰点(-17.45±0.89)℃最低;4龄幼虫的过冷却点(-15.02±0.55)℃和体液结冰点(-11.80±0.64)℃次之;2日龄雄成虫的过冷却点(-14.74±0.42)℃和体液结冰点(-11.26±0.42)℃与3龄幼虫的过冷却点(-14.07±0.68)℃和体液结冰点(-11.25±0.61)℃差异不显著;2日龄雌成虫的过冷却点(-12.97±0.40)℃和体液结冰点(-9.65±0.32)℃最高。结果显示,多异瓢虫蛹的耐寒性最强,2日龄雄成虫的耐寒性高于雌成虫,2日龄雌成虫的耐寒性最弱。Hippodamia variegata is the dominant predatory natural enemy of cotton fields in Xinjiang.In order to clarify the cold tolerance of Hippodamia variegata at different developmental stages,we determined the supercooling point and freezing points of hymolymph in 3rd-instar,4th-instar larvae,pupae,2-day-old female and male adults by using the supercooling point instrument,and then analyzed their frequency distribution.The results showed that the supercooling point(-21.05±0.75)℃and freezing point(-17.45±0.89)℃of pupae were the lowest among the larvae,pupae and adults,followed by the supercooling point(-15.02±0.55)℃and the freezing point(-11.80±0.64)℃of the 4th-instar larvae.The supercooling point(-14.74±0.42)℃and freezing point(-11.26±0.42)℃of 2-day-old male adults were not significant different with those of the 3rd-instar larvae(-14.07±0.68)℃and(-11.25±0.61)℃,respectively.The 2-day-old female adults had the highest supercooling point(-12.97±0.40)℃and freezing point(-9.65±0.32)℃.Our data indicated that the pupa of H.variegata had the strongest cold tolerance,and the 2-day-old male adults had higher cold tolerance than that of the female adult,and the 2-day-old female adults showed the weakest cold tolerance among the larvae,pupae and adults.

关 键 词：多异瓢虫 过冷却点 结冰点 抗寒性 

分 类 号：S476.2[农业科学—农业昆虫与害虫防治]