食管癌高发人群血浆游离DNA中的p38MAPK基因检测研究  

Detection of p38MAPK gene in plasma cell-free DNA among patients with high incidence of esophageal cancer

作　　者：冯志寅 吴怡娴 哈晓丹 陈邬锦[2] 李秀梅[2] 董娟娟[2] FENG Zhiyin;WU Yixian;HA Xiaodan;CHEN Wujin;LI Xiumei;DONG Juanjuan(Department of Pathology,First Affiliated Hospital,Xinjiang Medical University,Urumqi 830011;College of Basic Medicine,Xinjiang Medical University,Urumqi 830011,Xinjiang,China)

机构地区：[1]新疆医科大学第一附属医院病理科,新疆乌鲁木齐830011 [2]新疆医科大学基础医学院,新疆乌鲁木齐830011

出　　处：《癌变．畸变．突变》2022年第2期105-109,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：新疆维吾尔自治区自然科学基金(2018D01C175);省部共建中亚高发病成因与防治国家重点实验室开放课题基金(SKL-HIDCA-2018-26)。

摘　　要：目的:检测食管癌高发人群哈萨克族食管癌患者血浆游离DNA(cfDNA)中的p38MAPK基因并结合食管癌组织样本中p38MAPK蛋白的表达情况,探讨cfDNA中p38MAPK基因作为食管癌诊断新型分子标志物的意义。方法:收集20例哈萨克族食管癌患者手术治疗前、后的清晨空腹外周静脉血各10 mL及相对应患者术后组织的石蜡切片标本,10例健康哈萨克族人群的外周静脉血10 mL。离心柱吸附法提取cfDNA,直接PCR法检测cfDNA中的p38MAPK基因。免疫组织化学法检测患者术后组织中p38MAPK蛋白的表达情况并根据患者年龄、性别、分化程度、肿瘤最大径、T分期进行分组比较。结果:哈萨克族食管癌患者手术治疗前、后血液中cfDNA浓度均高于健康哈萨克族人群(P<0.05);患者手术治疗前cfDNA中p38MAPK基因检出率显著高于健康人群组(P<0.05),手术后cfDNA中p38MAPK基因检出率与健康人群组差异无统计学意义(P>0.05);患者术后免疫组化检测结果显示p38MAPK蛋白在高、中分化组中的阳性表达率高于低分化组(χ^(2)=13.939,P<0.05),在肿瘤最大径≤2.5 cm组中的阳性表达率高于>2.5 cm组(χ^(2)=0.014,P<0.05),p38MAPK蛋白的阳性表达率在不同年龄、性别、T分期患者肿瘤组织之间的表达差异无统计学意义(P>0.05)。结论:食管癌高发人群血液中cfDNA浓度显著高于健康人群,提示cfDNA浓度可能用于早期食管癌筛查。哈萨克族食管癌患者血液cfDNA中p38MAPK基因检出率显著高于健康人群组,又提示cfDNA中p38MAPK基因可能作为诊断食管癌的非侵入性新型分子标志物。OBJECTIVE:To detect the p38MAPK gene in cell-free DNA(cfDNA)samples among Kazak esophageal cancer patients with high incidence of esophageal cancer,and the p38MAPK protein in cancer tissues,to explore their values as new molecular markers for detecting esophageal cancers.METHODS:Fasting peripheral venous blood samples from 20 Kazak esophageal cancer patients were collected before and after they had surgical treatments,and paraffin sections from the corresponding patients'postoperative tissues were collected.Blood samples from 10 healthy Kazaks were collected as controls.The cfDNA samples were extracted by the centrifugation column adsorption method and used for detection of p38MAPK via direct PCR.Expressions of p38MAPK protein in postoperative tissues were detected using immunohistochemistry and compared according to age,gender,degree of differentiation,maximum tumor diameter and T stage in clinicopathological report.RESULTS:The concentrations of cfDNA in blood of Kazakh with esophageal cancer before and after surgical resections were higher than that of healthy Kazakh(P<0.05).The detection rates of p38MAPK gene in cfDNA before operation were significantly higher than that in healthy population group(P<0.05).However,there was no significant difference in the detection rates of p38MAPK gene in cfDNA after surgical resection between the two groups(P>0.05).Postoperative immunohistochemical detections show that the positive expression rate of p38MAPK protein in highly and moderately differentiated groups was higher than that in poorly differentiated groups(χ^(2)=13.939,P<0.05).The positive expression rate in the group with maximum tumor diameter≤2.5 cm was higher than that in the group with maximum tumor diameter>2.5 cm(χ^(2)=0.014,P<0.05).There was no significant difference in the positive expression rates of p38MAPK protein between tumor tissues of patients with different age,sex and T stage(P>0.05).CONCLUSION:The concentrations of cfDNA in the blood of esophageal cancer patients were significantly higher than

关 键 词：食管癌 哈萨克族 血浆游离DNA P38MAPK 循环肿瘤DNA 

分 类 号：R734.2[医药卫生—肿瘤]