胸腔积液肺腺癌细胞中CXCR4、Ki67、MDM2、N-cadherin的表达及其与EGFR突变状态的关系  

CXCR4,Ki67,MDM2 and N-cadherin expressions in lung adenocarcinoma pleural effusion cells and their correlation with EGFR mutation status

作　　者：王蕊[1] 刘颖[1] 吴娟[1] 纪晓坤 马阳 郭晓 杜芸[1] WANG Rui;LIU Ying;WU Juan;JI Xiaokun;MA Yang;GUO Xiao;DU Yun(Cancer Detection Center,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China)

机构地区：[1]河北医科大学第四医院癌检中心,河北石家庄050011

出　　处：《癌变．畸变．突变》2022年第2期114-118,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：河北省财政厅政府资助专科能力建设和专科带头人培养项目(361006)。

摘　　要：目的:研究胸腔积液肺腺癌细胞中趋化因子受体4(CXCR4)、增殖细胞核抗原Ki67、鼠双微体2(MDM2)和N-钙黏蛋白(Ncadherin)的表达与表皮生长因子受体(EGFR)突变状态的相关性。方法:收集46例晚期肺腺癌患者的胸腔积液,采用HE染色联合免疫细胞化学染色(ICC)法检测胸腔积液中是否含肿瘤细胞,突变扩增系统(ARMS)检测胸腔积液中癌细胞的EGFR基因突变状态,将癌细胞分为EGFR-TKI敏感突变组和EGFR野生型组。采用ICC法检测胸腔积液中肺腺癌细胞CXCR4、Ki67、MDM2和Ncadherin的表达,分析这些蛋白的表达阳性率和EGFR突变状态的相关性。结果:46例肺腺癌患者的恶性胸腔积液细胞中,31例(67.4%)存在EGFR-TKI敏感突变。CXCR4、Ki67、MDM2、N-cadherin表达阳性率与EGFR突变状态相关(r分别为0.452、0.428、0.417、0.524,均为P<0.05),且EGFR-TKI敏感突变组细胞CXCR4、Ki67、MDM2、N-cadherin表达的阳性率明显高于EGFR野生型组(P<0.05)。结论:EGFR突变状态与CXCR4、Ki67、MDM2和N-cadherin的表达相关,EGFR突变状态下,胸腔积液肺腺癌细胞的CXCR4、Ki67、MDM2和N-cadherin的表达阳性率高于非突变状态下的表达阳性率。OBJECTIVE:To explore correlations between EGFR mutation status and expressions of CXCR4,Ki67,MDM2 and N-cadherin in lung adenocarcinoma cells from pleural effusion.METHODS:Tumor cells from pleural effusions of lung adenocarcinomas were diagnosed using HE staining and immunocytochemistry(ICC).EGFR gene mutation status of lung adenocarcinoma patients was detected by the amplification refractory mutation system(ARMS)method,and divided into EGFR-TKI sensitive mutation group and EGFR wild-type group.Expressions of CXCR4,Ki67,MDM2 and N-cadherin were detected using the ICC technique,and the correlations between the expression levels of these proteins and EGFR mutation status were analyzed.RESULTS:In 46 patients with malignant pleural effusion,31 patients(67.4%)harbored EGFR-TKIsensitive mutations.The expression levels of CXCR4,Ki67,MDM2,and N-cadherin were correlated with EGFR mutation status(r=0.452,0.428,0.417,0.524,respectively,P<0.05).CONCLUSION:Expression levels of CXCR4,Ki67,MDM2 and N-cadherin were correlated with EGFR mutation status.Furthermore,expression levels of CXCR4,Ki67,MDM2 and N-cadherin in lung adenocarcinoma cells with pleural effusions were higher in tumors with EGFR mutation than without mutation.

关 键 词：胸腔积液 表皮生长因子受体 趋化因子受体4 细胞增殖核抗原Ki67 鼠双微体2 N-钙黏蛋白 

分 类 号：R734.2[医药卫生—肿瘤]