抵当芪桂汤对2型糖尿病大鼠肝组织PI3K、Akt基因和蛋白的影响  

Effects of Didang Qigui Decoction on PI3K and Akt Gene and Protein in Liver Tissue of Type 2 Diabetic Rats

作　　者：韩思荣 屈杰[1] 杨景锋[1] 杨军[1] 谭颖颖[1] 李小会[1] 陈丽名[1] HAN Sirong;QU Jie;YANG Jingfeng;YANG Jun;TAN Yingying;LI Xiaohui;CHEN Liming(Shaanxi University of traditional Chinese medicine,Shaanxi Xianyang 712046,China)

机构地区：[1]陕西中医药大学,陕西咸阳712046

出　　处：《现代中医药》2022年第2期20-24,共5页Modern Chinese Medicine

基　　金：陕西省教育厅科研项目(19JK0227);伤寒学与经方辨治疑难病一流创新团队(2019-QN04);陕西省伤寒学与经方治疗辨治疑难病研究室项目。

摘　　要：目的研究抵当芪桂汤对2型糖尿病大鼠肝脏PI3K、Akt基因和蛋白的影响。方法采用高糖高脂饲料联合腹腔注射小剂量的链脲佐菌素建立2型糖尿病模型大鼠,随机分为模型组(Model组)、抵当芪桂汤高剂量组(DQG组)、抵当芪桂汤常量组(DQZ组)、二甲双胍组(Met组)、抵当芪桂汤加二甲双胍组(DQZX组)。灌胃6周后,测定大鼠空腹血糖(FBG)、糖化血红蛋白(HbA1c)和肝组织胰岛素水平;采用RT-PCR法检测肝组织PI3K、AktmRNA的表达;Western-blot法检测肝组织PI3K、Akt蛋白的表达。结果抵当芪桂汤常量组、抵当芪桂汤加二甲双胍组大鼠的FBG、HbA1c和肝组织胰岛素水平均呈现不同程度降低(P<0.05或P<0.01),肝组织中PI3K、Akt基因与蛋白的表达均显著上调(P<0.01)。结论抵当芪桂汤可能通过调控2型糖尿病大鼠肝脏胰岛素信号通路的关键分子PI3K、Akt的表达,调节糖代谢紊乱,改善胰岛素抵抗状态。Objective To study the effects of Didang Qigui decoction on PI3K and Akt gene and protein in liver of type 2 diabetic rats.Methods High glucose and high-fat diet combined with intraperitoneal injection of small dose of streptozotocin were used to establish type 2 diabetes mellitus rats.They were randomly divided into model group,high dose group of Didang Qigui decoction(group DQG),Didang Qigui decoction constant group(group DQZ),metformin group(group Met),Didang Qigui decoction plus metformin group(DQZX group).After 6 weeks of gavage,the levels of fasting blood glucose(FBG),glycosylated hemoglobin(HbA1c)and liver insulin were measured;RT-PCR was used to detect the expression of PI3K and Akt mRNA in liver tissue;The expression of PI3K and Akt protein in liver tissue was detected by western blot.Results The levels of FBG,HbA1c and insulin in liver tissue decreased in different degrees in Didang Qigui decoction constant group and Didang Qigui decoction plus metformin group(P<0.05 or P<0.01),and the expressions of PI3K and Akt genes and proteins in liver tissue increased significantly(P<0.01).Conclusion Didang Qigui decoction can regulate the expression of PI3K and Akt in the insulin signaling pathway of type 2 diabetic rats,regulate glucose metabolism disorder and improve insulin resistance.

关 键 词：抵当芪桂汤 2型糖尿病 PI3K/AKT 肝脏 胰岛素抵抗 

分 类 号：R259[医药卫生—中西医结合]