The antigenicity of SARS-CoV-2 Delta variants aggregated 10 high-frequency mutations in RBD has not changed sufficiently to replace the current vaccine strain  

作　　者：Jiajing Wu Jianhui Nie Li Zhang Hao Song Yimeng An Ziteng Liang Jing Yang Ruxia Ding Shuo Liu Qianqian Li Tao Li Zhimin Cui Mengyi Zhang Peng He Youchun Wang Xiaowang Qu Zhongyu Hu Qihui Wang Weijin Huang 

机构地区：[1]Division of HIV/AIDS and Sex-transmitted Virus Vaccines,Institute for Biological Product Control,National Institutes for Food and Drug Control(NIFDC),102629 Beijing,China [2]Research Network of Immunity and Health(RNIH),Beijing Institutes of Life Science,Chinese Academy of Sciences,100101 Beijing,China [3]CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Center for Influenza Research and Early-warning(CASCIRE),CAS-TWAS Center of Excellence for Emerging Infectious Diseases(CEEID),Chinese Academy of Sciences,100101 Beijing,China [4]Division of Hepatitis and Enteric Viral Vaccines,Institute for Biological Product Control,National Institutes for Food and Drug Control(NIFDC)and Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products,102629 Beijing,China [5]Translational Medicine Institute,First People’s Hospital of Chenzhou,University of South China,Chenzhou,China [6]CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,100101 Beijing,China

出　　处：《Signal Transduction and Targeted Therapy》2022年第2期492-501,共10页信号转导与靶向治疗（英文）

基　　金：This work was supported by the General Program of the National Natural Science Foundation of China(grant number 82073621&82172244);National Key Research and Development Program of China(grant number 2021YFC0863300);Beijing Municipal Science and Technology Project(Z211100002521018);Bill&Melinda Gates Foundation(Investment ID INV-006379).

摘　　要：Emerging SARS-CoV-2 variants are the most serious problem for COVID-19 prophylaxis and treatment.To determine whether the SARS-CoV-2 vaccine strain should be updated following variant emergence like seasonal flu vaccine,the changed degree on antigenicity of SARS-CoV-2 variants and H3N2 flu vaccine strains was compared.The neutralization activities of Alpha,Beta and Gamma variants’spike protein-immunized sera were analysed against the eight current epidemic variants and 20 possible variants combining the top 10 prevalent RBD mutations based on the Delta variant,which were constructed using pseudotyped viruses.Meanwhile,the neutralization activities of convalescent sera and current inactivated and recombinant protein vaccine-elicited sera were also examined against all possible Delta variants.Eight HA protein-expressing DNAs elicited-animal sera were also tested against eight pseudotyped viruses of H3N2 flu vaccine strains from 2011–2019.Our results indicate that the antigenicity changes of possible Delta variants were mostly within four folds,whereas the antigenicity changes among different H3N2 vaccine strains were approximately 10–100-fold.Structural analysis of the antigenic characterization of the SARS-CoV-2 and H3N2 mutations supports the neutralization results.This study indicates that the antigenicity changes of the current SARS-CoV-2 may not be sufficient to require replacement of the current vaccine strain.

关 键 词：vaccine ANTIGENICITY VARIANTS 

分 类 号：R51[医药卫生—内科学]