基于OSMAC策略对海洋来源红树林内生真菌FYF J-9化学成分多样性的初步研究  

Preliminary Study on the Chemical Diversity of Mangrove Endophytic Fungus FYF J-9 based on OSMAC Strategy

作　　者：陈月清 王丽霞 刘付倩 周俊强 黄纪国 Chen Yueqing;Wang Lixia;Liu Fuqian;Zhou Junqiang;Huang Jiguo(College of food and Biotechnology,Guangdong Industry Poytechnic,Guangzhou 510300,China)

机构地区：[1]广东轻工职业技术学院轻化工技术学院,广东广州510300

出　　处：《广东化工》2022年第5期15-17,52,共4页Guangdong Chemical Industry

基　　金：广州市科技基础研究计划基础与应用基础研究项目(202102020608);广东轻工职业技术学院挑战杯。

摘　　要：本文研究OSMAC策略下海洋真菌FYF J-9化学成分多样性。采用正交实验方法考察不同培养基、海水盐度和培养时间等因素对红树林内生真菌FYF J-9次级代谢产物的影响。实验结果表明:影响次级代谢产物产量因素依次为:培养基>海水盐度>培养时间,综合微生物外观分析与TLC指纹图谱分析,最终确定红树林内生真菌FYF J-9最佳发酵培养条件分别为大米培养基(33.3 g/L、20 d)。In this study, the one strain many compounds(OSMAC) strategy was confirmed the chemical diversity of mangrove endophytic fungus FYF J-9. The optimal fermentation conditions of the strain were performed by adjusting the culture conditions under different medium, salinity and culture time. The results showed that the factors affecting the yield of secondary metabolites were as follows: culture medium>seawater salinity>culture time. Finally, the optimal fermentation conditions of mangrove endophytic fungus FYF J-9 were with rice medium in salinity 33.3 g/L(20 d) on the basis of microbial fermentation appearance analysis and TLC fingerprint analysis.

关 键 词：OSMAC策略 红树林内生真菌 次生代谢产物 曲霉属 TLC指纹图谱分析 

分 类 号：TQ[化学工程]