基于棕榈仁油的特医脂肪乳液制备及其对小鼠血脂的影响  

Preparation of Special Medical Fat Emulsion Based on Palm Kernel Oil and its Effect on Blood Lipid in Mice

作　　者：朱海华 葛瑞宏 马银辉 杨峻豪 王永 王慧[2] ZHU Hai-hua;GE Rui-hong;MA Yin-hui;YANG Jun-hao;WANG Yong;WANG Hui(Henan Commerce Science Institute Co.,Ltd.,Zhengzhou,Henan 450002,China;School of Public Health,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Palm Oil Research and Technical Service Institute of Malaysian Palm Oil Board,Shanghai 201108,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]河南省商业科学研究所有限责任公司,河南郑州450002 [2]上海交通大学医学院公共卫生学院,上海200025 [3]马来西亚棕榈油总署大马棕榈油技术研发(上海)有限公司,上海201108 [4]上海海洋大学食品学院,上海201306

出　　处：《粮油食品科技》2022年第2期85-94,共10页Science and Technology of Cereals,Oils and Foods

基　　金：马来西亚棕榈油总署基金项目(PORTSIM 068/2019)。

摘　　要：中链甘油三酯是特医脂肪组件的重要组成成分,棕榈仁油是其天然食物来源。为开发基于棕榈仁油的特医脂肪乳液产品,依据《特殊医学用途配方食品通则》《中国居民膳食营养素参考摄入量》等进行脂肪酸均衡型乳液油相配方设计,并分析其脂肪酸组成;以Zeta电位、粒径为指标优化脂肪乳液制备工艺,并对研发产品进行稳定性评价,通过小鼠试验研究其对血清血脂的影响。结果表明,油相不饱和脂肪酸中n–6与n–3比值为5.16±0.05;采用分步乳化法,最佳乳化工艺为乳化速度10000 r/min,乳化时间20 min,乳化温度50℃;初乳液经高压均质机50 MPa均质三次,121℃高压灭菌10 min,在上述条件下制备的脂肪乳液产品质量稳定。动物试验结果表明,该脂肪酸均衡型脂肪乳液具有显著降低小鼠血清甘油三酯(Triglycerides,TG)含量(P<0.01)、降低血清总胆固醇(Total cholesterol,TC)含量(P<0.01),以及降低低密度脂蛋白胆固醇(Low-density lipoprotein cholesterol,LDL-C)含量(P<0.05)和升高高密度脂蛋白胆固醇(High-density lipoprotein cholesterol,HDL-C)含量(P<0.01)的作用,对于调节血脂水平具有一定意义。MCTs is an important component of fat emulsion for special medical purposes(FESMP),and palm kernel oil is the natural food source of MCTs.In order to develop palm kernel oil based FESMP,the oil phase formula of the emulsion was designed according to the General Rules of Formula Food for Special Medical Use and the Reference Dietary Nutrient Intake of Chinese Residents,and the fatty acid composition was analyzed.The preparation process of fat emulsion was optimized by Zeta potential and particle size,the stability was evaluated and the effect on serum lipid was studied in mice.The results showed that the ratio of n-6 to n-3 in oil phase was 5.16±0.05;Step-by-step emulsification method was adopted,and the optimal emulsification process parameters were as follows:emulsification speed of 10000 r/min,emulsification time of 20 min,and emulsification temperature of 50℃.The initial emulsion was homogenized by high pressure homogenizer at 50 MPa for three times and autoclaved at 121℃for 10 min,and the product quality was stable.The results of animal experiments showed that the fatty acid balanced emulsion could significantly reduce the content of Triglycerides(TG)in serum of mice(P<0.01),and reduce the content of Total cholesterol(TC)in serum(P<0.01).It can also reduce the Low-density lipoprotein cholesterol,LDL-C content(P<0.05)and increase the content of High-density lipoprotein cholesterol(HDL-C)(P<0.01).Therefore,it has a certain significance for regulating the level of blood lipid.

关 键 词：中链甘油三酯 棕榈仁油 脂肪酸均衡 乳化工艺 特医脂肪乳液 调节血脂 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]