狗肝菜多糖干预MAPK信号通路抑制炎症反应缓解大鼠肝纤维化进程  

Study on intervention of DCP on MAPK signaling pathway to inhibit inflammatory response and alleviate progression of liver fibrosis in rats

作　　者：徐杰 钟明利[1] 王跃峰[1] 李波[1] 韩佳佳 高雅[1] 张可锋[1] XU Jie;ZHONG Ming-li;WANG Yue-feng;LI Bo;HAN Jia-jia;GAO Ya;ZHANG Ke-feng(Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院,广西桂林541199

出　　处：《中国药理学通报》2022年第4期531-537,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金项目(No 81960779,81760114);广西自然科学基金(No 2020GXNFSBA297005,2018GXNSFAA281040)。

摘　　要：目的探究狗肝菜多糖(dicliptera chinensis polysaccharide,DCP)对2,4-二甲基亚硝胺(2,4-dimethylnitrosamine,DMN)诱导的肝纤维化(hepatic fibrosis,HF)大鼠模型是否具有保护作用以及对MAPK信号通路的影响。方法大鼠通过腹腔注射DMN以建立HF模型,造模大鼠随机分为5组,包括模型组、秋水仙碱组、DCP低、中、高剂量组,另设对照组,连续给药6周。6周后取大鼠血清检测肝功能生化指标;取肝组织进行HE、Masson染色和免疫组化实验;RT-PCR检测炎症因子的表达;Western blot法检测MAPK通路相关蛋白的表达,观察DCP对HF的预防作用,并探索其对MAPK通路的干预作用。结果模型组大鼠肝功能严重受损,出现明显的肝细胞损伤、炎性细胞浸润及间质纤维化增多,提示HF模型制备成功;各给药组较模型组可明显降低HF的病变程度、减少胶原沉积和炎症因子的表达,明显下调MAPK通路蛋白的表达。结论DCP可干预MAPK信号通路抑制炎症反应缓解大鼠HF进程。Aim To investigate whether DCP has protective effect on 2,4-dimethylnitrosamine(DMN)-induced liver fibrosis rat model and its effect on MAPK signaling pathway.Methods Rats were intraperitoneally injected with DMN to establish HF model,and then were randomly divided into five groups,namely model group,colchicine group,DCP low-dose,medium-dose and high-dose groups,and control group.The rats were given DMN continuously for six weeks.Serum was collected afterwards to detect biochemical indexes of liver function.HE and Masson staining and immunohistochemical experiments were performed on liver tissues.RT-PCR was applied to detect the expression of inflammatory factors.Western blot was used to detect the expression of proteins related to MAPK pathway,the preventive effect of DCP on HF was observed,and its intervention effect on MAPK pathway was explored.Results The liver function of rats in model group was severely impaired,with obvious hepatocyte damage,inflammatory cell infiltration and increased interstitial fibrosis,suggesting that the preparation of HF model was successful.Conclusions DCP can interfere with MAPK signaling pathway to inhibit the inflammatory response and alleviate the progression of HF in rats.

关 键 词：狗肝菜多糖 2 4-二甲基亚硝胺 肝纤维化 炎症因子 MAPK通路 药效机制 

分 类 号：R-332[医药卫生] R284.1