咪喹莫特诱导银屑病模型小鼠T细胞免疫功能改变与性别的关系  

Imiquimod-induced changes in T cell immune function of mice with psoriasis and the relationship between sex

作　　者：杨梅 唐彩红 桂雨晴 陈镜宇[1] 魏伟[1] YANG Mei;TANG Cai-hong;GUI Yu-qing;CHEN Jing-yu;WEI Wei(Institute of Clinical Pharmacology, Anhui Medical University, Key Lab of Anti-inflammatory and Immune Medicine of Education Ministry, Anhui Cooperative Innovation Center of Anhuiinflammatory Immune Drugs, Hefei 230032, China)

机构地区：[1]安徽医科大学临床药理研究所,抗炎免疫药物教育部重点实验室,安徽抗炎免疫药物协同创新中心,安徽合肥230032

出　　处：《中国药理学通报》2022年第4期589-597,共9页Chinese Pharmacological Bulletin

基　　金：安徽省高校自然科学研究重点项目(No KJ2019A0263)。

摘　　要：目的研究咪喹莫特诱导银屑病模型小鼠T细胞免疫功能改变及角质细胞损伤与性别的关系。方法将IMQ诱导的银屑病小鼠随机分为雌、雄模型组,设雌、雄正常组涂抹等量凡士林。银屑病皮损面积和严重程度(psoriasis area and severity index,PASI)评分评价皮损病变程度;HE染色观察皮损皮肤病理变化;Western blot和免疫荧光检测皮损皮肤增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、Ki67、角蛋白1(keratin 1,K1)、角蛋白10(keratin 10,K10)及involucrin蛋白水平;计算脾脏指数;免疫组化检测皮损皮肤和脾脏中CD4、IFN-γ、IL-4以及IL-17的表达;流式细胞术检测脾脏中Th1、Th17细胞亚群变化。结果与雌、雄正常组相比,雌、雄模型组PASI评分增加,皮损皮肤异常增厚与分化,PCNA、Ki67增加,K1、K10及involucrin减少,脾脏指数增大,皮损皮肤及脾脏中CD4、IFN-γ、IL-17水平升高,IL-4的水平降低,脾脏中Th1以及Th17细胞亚群增加。而雌、雄模结论性别对IMQ诱导的银屑病样小鼠T细胞免疫功能的异常活化及角质细胞的增殖、分化没有影响。Aim To research the effect of gender on immune function and keratinocyte damage in imiquimod(IMQ)induced psoriasis mice.Methods IMQ-induced psoriasis mice were freely divided into female and male model groups,and female and male normal groups were set up smeared with an equal amount of petroleum.PASI score and HE staining were used to evaluate skin lesion and pathology;Western blot and immunity fluorescence were used to detect the expression of proliferating cell nuclear antigen(PCNA),Ki67,keratin 1(K1),keratin 10(K10),and involucrin in skin lesions;spleen index of mice was calculated;immunohistochemistry was used to detect the expression of CD4,IFN-γ,IL-4,and IL-17 in skin and spleen;flow cytometry was used to detect the changes of Th1 and Th17 cell subsets in spleen.Results Compared with female and male normal groups,PASI score of female and male model groups increased,skin lesions were abnormally thickened and differentiated,the level of PCNA and Ki67,the spleen index,the Th1 and Th17 cell subsets in the spleen both increased,K1,K10,and involucrin decreased,the levels of CD4,IFN-γ,IL-17 in skin lesions and spleen were elevated,but the level of IL-4 showed the opposite trend.There was no statistical difference in the above indicators between the female and male model groups.Conclusion Gender has no effect on the abnormal activation of T cell immune function and the proliferation and differentiation of keratinocytes in psoriasis-like mice induced by IMQ.

关 键 词：银屑病 咪喹莫特 角质细胞 增殖分化 T细胞免疫 性别 

分 类 号：R-332[医药卫生] R322.991