基于网络药理学探讨健脾补肾活血方治疗缺血性卒中的作用机制  

Network pharmacology study on mechanism of Jianpi Bushen Huoxue Prescription in treatment of ischemic stroke

作　　者：李梦君 乔战科 刘向哲[2] 孙永康 冉春龙 白琛 方晓艳[3] LI Meng-jun;QIAO Zhan-ke;LIU Xiang-zhe;SUN Yong-kang;RAN Chun-long;BAI Chen;FANG Xiao-yan(Henan University of Chinese Medicine, Zhengzhou 450046,China;Neurology First Ward of The Fist Affiliated, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000,China;School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南中医药大学第一附属医院脑病一区,河南郑州450000 [3]河南中医药大学药学院,河南郑州450046

出　　处：《中国药理学通报》2022年第4期605-612,共8页Chinese Pharmacological Bulletin

基　　金：河南省重点研发与推广专项(科技攻关)(No 212102310360);国家中医临床研究基地科研专项课题(No 2019JDZX2008);河南省中医药科学研究专项课题(No 2019ZY2031);河南省中医药传承与创新人才工程(仲景工程)中医药学科领军人才:豫卫中医函【2021】8号。

摘　　要：目的运用网络药理学方法分析健脾补肾活血方治疗缺血性卒中的作用机制,进一步从分子生物学层面为该方实验研究和临床应用提供理论基础。方法根据TCMSP数据库、5种疾病数据库查找药物及IS相关靶点,应用Cytoscape 3.8.0和String平台分别进行关键化学成分和核心靶点的筛选,并构建PPI网络图;通过DAVID平台进行GO功能分析和KEGG通路分析,使用分子对接技术进行核心成分与关键靶点验证。结果健脾补肾活血方治疗缺血性卒中的有效成分可能为槲皮素、木犀草素、山柰酚、丹参酮IIA等;潜在结论该方多组分、多靶点及多途径治疗缺血性卒中。Aim To analyze the mechanism of Jianpi Bushen Huoxue Prescription in treating ischemic stroke by means of network pharmacology,and further provide theoretical basis for the experimental research and clinical application of this recipe from molecular biology level.Methods According to TCMSP database and five disease databases,the drug and ischemic stroke related targets were searched,and the key chemical components and core targets were screened by using Cytoscape 3.8.0 and String platform respectively,and the PPI network diagram was constructed;GO functional analysis and KEGG pathway analysis were performed on DAVID platform,and molecular docking technology was used to verify the core components and key targets.Results The effective components of Jianpi Bushen Huoxue Prescription in treating ischemic stroke may be quercetin,luteolin,kaempferol,tanshinone IIA,etc.The potential targets were STAT3,TP53,MAPK1,MAPK3,etc.The related pathways mainly included TNF signaling pathway,HIF-1 signaling pathway,PI3K-Akt signaling pathway,Toll-like receptor and so on.Molecular docking showed that the core compounds had good binding conformation with key targets.Conclusion This prescription is multi-component,multi-target and multi-channel in treating ischemic stroke.

关 键 词：网络药理学 健脾补肾活血方 缺血性卒中 信号通路 分子对接 机制 中医药 

分 类 号：R289.5[医药卫生—方剂学] R319[医药卫生—中药学]