光活化消毒技术清除小鼠舌部白色念珠菌的实验研究  

Experimental study of the removal of Candida albicans from mouse tongues using photoactivated disinfection

作　　者：谷悦 刘娜[2] 王佳宁 张立芳 刘庆[3] GU Yue;LIU Na;WANG Jianing;ZHANG Lifang;LIU Qing(Department of Pediatric Dentistry,School and Hospital of Stomatology,Hebei Medical University,Hebei Key Laboratory of Stomatology,Hebei Clinical Research Center for Oral Diseases,Shijiazhuang 050017,China;Department of Oral Prevention,School and Hospital of Stomatology,Hebei Medical University,Hebei Key Laboratory of Stomatology,Hebei Clinical Research Center for Oral Diseases,Shijiazhuang 050017,China;Centre of Experimentation,School and Hospital of Stomatology,Hebei Medical University,Hebei Key Laboratory of Stomatology,Hebei Clinical Research Center for Oral Diseases,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学口腔医学院·口腔医院儿童口腔科,河北省口腔医学重点实验室,河北省口腔疾病临床医学研究中心,河北石家庄050017 [2]河北医科大学口腔医学院·口腔医院口腔预防科,河北省口腔医学重点实验室,河北省口腔疾病临床医学研究中心,河北石家庄050017 [3]河北医科大学口腔医学院·口腔医院实验中心,河北省口腔医学重点实验室,河北省口腔疾病临床医学研究中心,河北石家庄050017

出　　处：《口腔疾病防治》2022年第6期406-411,共6页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：河北省科技计划项目(20377799D);河北省政府资助专科带头人培养项目(2018133206-2);河北省卫生健康委员会医学科学研究课题(20191079);河北省老年病防治项目(361029);河北医科大学大学生创新性实验项目(USIP2021209)。

摘　　要：目的 建立小鼠急性假膜型念珠菌性口炎模型,观察光活化消毒技术(photoactivated disinfection,PAD)在体内清除白色念珠菌的效果,初步探索该技术应用于急性假膜型念珠菌性口炎的可行性。方法 选取6周龄雄性ICR小鼠,向已产生免疫抑制的小鼠舌背接种浓度为1×10^(7)CFU/mL的白色念珠菌菌液,成功建立急性假膜型念珠菌性口炎模型30只,随机分为对照组、光活化组,每组15只。光活化组小鼠舌背部涂抹1 mg/mL甲苯胺蓝溶液,孵育1 min,750 mW LED红光照射1 min后即刻与对照组小鼠行舌背真菌载量测定,48 h后再次对两组小鼠行舌背真菌载量测定,并行舌部组织病理学检查。结果 PAD处理后48 h,光活化组舌背白色假膜明显少于对照组。PAD处理后即刻和48 h,光活化组舌背真菌载量均明显低于对照组,差异具有统计学意义(P <0.05)。PAD处理后48 h,光活化组经HE染色显示较对照组上皮结构更加规则,未见微小脓肿;经PAS染色显示菌丝数量明显少于对照组,偶见菌丝侵入角化层,但未深入上皮层。结论 PAD能显著清除急性假膜型念珠菌性口炎小鼠舌部的白色念珠菌。Objective To establish a mouse model of acute pseudomembranous stomatitis and to observe the effect of photoactivated disinfection(PAD) on the removal of Candida albicans in vivo, and initially explore the feasibility of this technology in the treatment of acute pseudomembranous stomatitis. Methods Six-week-old male ICR mice were selected and immunized with 1 × 10^(7)CFU/mL Candida albicans solution on the backs of the tongues of immunosuppressed mice. Thirty model mice with acute pseudomembranous stomatitis were successfully established and randomly divided into a control group and a photoactivated disinfection group, with 15 mice in each group. Mice in the photoactivated disinfection group were coated with 1 mg/mL toluidine blue solution on the back of the tongue, incubated for 1min and irradiated with 750 mW LED red light for 1 min. Immediately after treatment, the tongue fungal load was measured in the photoactivated disinfection group and the control group. Tongue fungal load was measured again 48 h later,and tongue histopathological examination was performed in both groups. Results Forty-eight hours after PAD treatment, the white pseudomembrane on the back of the tongue in the photoactivated group was significantly less than the control group. The fungal load on the dorsum of the tongue in the treatment group was significantly lower than the control group immediately and 48 h after treatment for PAD, and the difference was statistically significant(P < 0.05). Fortyeight hours after PAD treatment, HE staining showed that the epithelial structure of the PAD group was more regular than the control group, and no microabscesses were observed. PAS staining showed that the number of mycelia in the PAD group was significantly less than the control group. Mycelia occasionally invaded the keratinized layer but did not penetrate into the upper cortex. Conclusion PAD significantly removed Candida albicans from the tongues of mice with acute pseudomembranous Candida stomatitis.

关 键 词：光活化消毒技术 白色念珠菌 急性假膜型念珠菌性口炎 真菌载量 甲苯胺蓝溶液 小鼠 模型 实验研究 

分 类 号：R78[医药卫生—口腔医学]