大麦籽粒淀粉含量的QTL定位分析  

QTL Analysis of Grain Starch Content in Barley

作　　者：雍杨莹 于浪柳 李春燕 张蓝天 董梦圆 薛大伟[1] 方云霞 张晓勤[1] YONG Yangying;YU Langliu;LI Chunyan;ZHANG Lantian;DONG Mengyuan;XUE Dawei;FANG Yunxia;ZHANG Xiaoqin(College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,China)

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州311121

出　　处：《杭州师范大学学报（自然科学版）》2022年第2期158-163,共6页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31401316);浙江省大学生科技创新活动计划(新苗人才计划)项目(2020R427034).

摘　　要：以大麦H602和Golden Promise构建的134株重组自交系为材料,利用构建的高密度分子遗传图谱,对2个生长周期的籽粒直链淀粉含量和支链淀粉含量及总淀粉含量进行QTL定位分析.共检测到8个与淀粉含量相关的QTL位点.其中,直链淀粉含量相关QTL有2个,均位于染色体7H上,贡献率分别为8.52%和7.83%;支链淀粉含量相关QTL有3个,位于染色体2H和7H上,贡献率分别为15.53%、16.40%和12.71%;总淀粉含量相关QTL有3个,位于染色体2H和7H上,贡献率分别为24.05%、24.02%和12.79%.该结果为大麦品质育种提供了遗传基础.A total of 134 recombinant inbred lines(RILs)constructed by barley varieties H602 and Golden Promise were used as experimental materials,and the constructed high-density molecular genetic map was used to conduct QTL mapping analysis on amylose,amylopectin and total starch content in grains at two growth stages.Eight QTLs for starch content were detected.Among them,two QTLs related to amylose content were located on chromosome 7H,explaining 8.52%and 7.83%of the phenotypic variances,respectively.Three QTLs related to amylopectin content were located on chromosomes 2H and 7H,accounting for 15.53%,16.40%and 12.71%of the phenotypic variances,respectively.Three QTLs related to total starch content were located on chromosomes 2H and 7H,with 24.05%,24.02%and 12.79%of the variances,respectively.The results provide a genetic basis for barley quality breeding.

关 键 词：大麦 重组自交系 淀粉含量 QTL定位 

分 类 号：Q94[生物学—植物学]