变态幼蛙对蝌蚪时期农药胁迫的毒性响应  

Toxicological responses of metamorphosed juvenile frog to pesticide stress at the period of tadpoles

作　　者：杨淑玉 张蕊[1] 赵文阁[1] 于东[1] YANG Shu-yu;ZHANG Rui;ZHAO Wen-ge;YU Dong(Harbin Normal University,Harbin 150025,China)

机构地区：[1]哈尔滨师范大学,哈尔滨150025

出　　处：《生态学杂志》2022年第3期503-511,共9页Chinese Journal of Ecology

基　　金：哈尔滨师范大学博士启动资金(220601085)资助。

摘　　要：本研究探讨阿特拉津和毒死蜱染毒东北林蛙蝌蚪对变态幼蛙肝脏组织抗氧化酶、解毒酶、细胞色素P4503A(CYP3A)mRNA的影响。实验通过二者梯度剂量,单独、共同胁迫蝌蚪,变态完成后分析了肝脏组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、乙酰胆碱酯酶(AChE)、羧酸酯酶(CbE)及CYP3A mRNA表达的响应。结果表明:东北林蛙肝脏组织中的抗氧化酶(SOD,CAT)活性随农药剂量的上升而显著下降,解毒酶(AChE,CbE)随农药剂量的上升而明显下降,CYP3A mRNA相对表达显著下降。结果证实,该两种农药单独或共同染毒蝌蚪会对变态后东北林蛙产生毒性作用,使东北林蛙抗氧化酶、解毒酶活性和CYP3A mRNA相对表达发生显著变化,CYP3A mRNA相对表达与抗氧化酶水平和解毒酶水平的变化相同。We investigated the changes of the expression of antioxidant enzymes and detoxification enzymes and cytochrome P4503 A(CYP3A)in liver tissue of Rana dybowskii after exposure to atrazine and chlorpyrifos in tadpoles stage.Experiments were carried out under different concentrations of atrazine and chlorpyrifos,either alone or combined.The activities of catalase(CAT),superoxide dismutase(SOD),acetylcholinesterase(AChE),carboxylesterase(CbE),and relative expression of CYP3A mRNA were measured in the liver tissue of Rana dybowskii at the period of metamorphosis completion.The results showed that the activities of antioxidant enzymes(SOD and CAT)and detoxification enzymes(AchE and CbE)and relative expression of CYP3A mRNA in the liver tissue were significantly decreased with increasing pesticide concentrations.These results suggested that exposure of to single and combined pesticides at tadpole stage could make Rana dybowskii toxic at the period of metamorphosis completion,and significantly changed the activities of antioxidant enzymes and detoxification enzymes,and relative expression of CYP3A mRNA.The expression of CYP3A mRNA showed similar responses with the levels of antioxidant enzymes and detoxification enzymes.

关 键 词：东北林蛙 抗氧化酶 CYP3A 阿特拉津 毒死蜱 

分 类 号：X171.5[环境科学与工程—环境科学]