线粒体DNA D-环区单核苷酸多态性与胃癌发病年龄的关系研究  

Single Nucleotide Polymorphisms in the Mitochondrial Displacement Loop and Age at Onset of Gastric Cancer

作　　者：王英南[1] 吴忱思[1] 赵乐 张风宾[1] 张韶辰 郭占军[3] 张瑞星[1] WANG Yingnan;WU Chensi;ZHAO Yue;ZHANG Fengbin;ZHANG Shaochen;GUO Zhanjun;ZHANG Ruixing(Department of Gastroenterology,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China;Office of Educational Administration,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China;Department of Rheumatology and Immunology,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第四医院消化内科,河北省石家庄市050011 [2]河北医科大学第四医院教务处,河北省石家庄市050011 [3]河北医科大学第四医院风湿免疫科,河北省石家庄市050011

出　　处：《中国全科医学》2022年第15期1883-1887,共5页Chinese General Practice

基　　金：河北省卫生和计划生育委员会科研基金项目(20210508);河北省医学科学研究重点课题计划(21377759D)。

摘　　要：背景胃癌发病率和死亡率长期居高不下,防控形势严峻,且发病年龄逐渐年轻化,但目前关于胃癌发病年龄预测因素的研究报道较少。目的探讨线粒体DNA D-环区(D-Loop区)单核苷酸多态性(SNPs)与胃癌患者发病年龄之间的关系。方法选取2007年7月至2008年12月在河北医科大学第四医院消化内科经胃镜病理证实的胃癌患者150例为研究对象,提取胃癌患者外周血中的线粒体DNA,采用聚合酶链反应(PCR)扩增目的片段,并进行线粒体DNA D-Loop区测序。采用Kaplan-Meier法绘制胃癌患者发病年龄的生存曲线,比较采用Log-rank检验;采用多因素Cox比例风险回归分析探究胃癌患者发病年龄的影响因素。结果不同分化程度患者发病年龄比较,差异有统计学意义(P<0.05)。Log-rank检验结果显示,153G基因型患者的发病年龄〔(48.0±5.3)岁〕早于153A基因型患者〔(60.1±0.8)岁〕(χ^(2)=7.757,P=0.005)。多因素Cox比例风险回归分析结果显示,线粒体DNA D-Loop区SNPs位点153A/G是预测胃癌患者发病年龄的影响因素〔HR=0.323,95%CI(0.140,0.745),P=0.008〕。结论线粒体DNA D-Loop区SNPs位点153A/G或许可以作为胃癌发病年龄的新型预测指标,通过分析线粒体DNA D-Loop区的多态性,可以帮助识别早发的胃癌患者。Background It is very difficult to contain persistently high incidence and mortality rates of gastric cancer.Moreover,the age at onset of gastric cancer is becoming earlier.However,there are few studies on the prediction of the age at gastric cancer onset.Objective To investigate the relationship between single nucleotide polymorphisms(SNPs)in mitochondrial displacement loop(D-Loop)and age at gastric cancer onset.Methods A total of 150 patients with confirmed gastric cancer by pathologically examination of biopsy samples taken during gastroscopy were recruited from Department of Gastroenterology,the Fourth Hospital of Hebei Medical University between July 2007 and December 2008.PCR was used to amplify the targeted fragment of peripheral blood mitochondrial DNA,and the mitochondrial D-Loop region was sequenced.Survival curves for patients with different ages of onset of gastric cancer were plotted by the Kaplan-Meier method,and compared by the Log-rank test.Cox regression analysis was used to identify factors associated with age of onset of gastric cancer.Results The age of onset of patients with different degrees of education was statistically significant(P<0.05).Log-rank test revealed that patients with 153G genotype had earlier age of onset of gastric cancer than did those with 153A genotype〔(48.0±5.3)vs(60.1±0.8)〕(χ^(2)=7.757,P=0.005).Multivariate Cox regression analysis indicated that the 153A/G polymorphism in mitochondrial D-Loop was a predictor of age at onset of gastric cancer〔HR=0.323,95%CI(0.140,0.745),P=0.008〕.Conclusion The 153A/G polymorphism in mitochondrial D-Loop may be used as a novel predictor of the age at onset of gastric cancer.Analyzing the SNPs in mitochondrial D-Loop may contribute to early identification of gastric cancer.

关 键 词：胃肿瘤 DNA 线粒体 D-LOOP 多态性 单核苷酸 发病年龄 性别因素 

分 类 号：R735.2[医药卫生—肿瘤]