6-羟基多巴胺单侧帕金森病模型大鼠丘脑腹内侧核的放电活动  

Alterations of firing activity of the ventromedial thalamic nucleus in rats with unilateral 6-OHDA lesions

作　　者：潘琪 张旺明[2] 罗非[3] 徐如祥 PAN Qi;ZHANG Wangming;LUO Fei;XU Ruxiang(不详;Department of Neurosurgery,the First Affiliated Hospital,Hainan Medical University,Haikou 570102,China)

机构地区：[1]海南医学院第一附属医院神经外科,海口570102 [2]南方医科大学珠江医院神经外科,广州510280 [3]中国科学院心理研究所,北京100101 [4]四川省人民医院神经外科,成都610072

出　　处：《实用医学杂志》2022年第3期324-329,共6页The Journal of Practical Medicine

基　　金：海南省高等学校科学研究项目(编号:Hnky-2021-38);海南省卫生计生行业科研项目(编号:20A200319);海南省自然科学基金高层次人才项目(编号:821RC698)。

摘　　要：目的研究6-羟基多巴胺(6-OHDA)单侧帕金森病模型大鼠的丘脑腹内侧核神经元的放电活动。方法使用大鼠脑立体定位仪把记录电极尖端置入SD大鼠双侧的丘脑腹内侧核,把用于后期注射6-OHDA的不锈钢管一端置入右侧内侧前脑束的上方。向右侧内侧前脑束内注入12μg/4μL的6-OHDA制作单侧帕金森病大鼠模型。采用神经元单位放电在体多通道同步记录方法记录模型建立前和模型建立后的双侧丘脑腹内侧核神经元的动作电位。应用酪氨酸羟化酶免疫组织化学染色观察黑质内多巴胺能神经元的丧失情况。应用甲酚紫染色对记录电极尖端进行定位。结果模型大鼠的左侧和右侧丘脑腹内侧核神经元的平均放电频率分别是(15.5287±0.57269)Hz和(5.6981±0.2791)Hz,两者的差异有统计学意义(t=15.431,P<0.001)。与模型建立前比较,模型建立后,大鼠的右侧丘脑腹内侧核神经元的平均放电频率显著降低(F=306.701,P<0.001)。模型建立前和模型建立后的右侧丘脑腹内侧核神经元爆炸放电率分别是14.8%和44.4%。与模型建立前相比,模型建立后,大鼠的右侧丘脑腹内侧核中爆发放电神经元的比例明显增加,差异有统计学意义(P<0.001)。与左侧丘脑腹内侧核相比较,模型大鼠右侧丘脑腹内侧核中爆发放电神经元比例明显增加,差异有统计学意义(χ^(2)=8.355,P=0.004)。结论6-OHDA单侧帕金森病模型大鼠丘脑腹内侧核神经元放电活动显著改变,丘脑腹内侧核可能在帕金森病的病理生理机制中扮演重要角色。Objective To explore the firing activity of the ventromedial thalamic nucleus(VM)in rats with unilateral 6-OHDA lesions.Methods The recording electrodes were implanted in the bilateral ventromedial thalamic nucleus of SD rats via stereotactic surgery,and the stainless steel cannula for 6-OHDA injection was implanted above the right medial forebrain bundle.The unilateral Parkinson’s disease rat model was induced by injection of 12μg/4μL 6-OHDA into the right medial forebrain bundle.Extracellular signals in the bilateral VM were recorded in vivo using multi-channel methods before and after lesion.Tyrosine hydroxylase immunohistochemical staining was used to detect the damage of dopaminergic neurons in substantianigra.Cresol violet staining was used to determine the positions of the tips of the recording electrodes.Results In model rats,the mean firing rates of neurons in the left VM and the right VM were(15.5287±0.57269)Hz and(5.6981±0.2791)Hz,respectively,with significant difference(t=15.431,P<0.001).Compared with that before the establishment of the model,the mean firing rates of neurons in the right VM decreased significantly after the establishment of the model(F=306.701,P<0.001).Before and after the model’s establishment,the rates of burst firing neurons in the right VM were 14.8%and 44.4%,respectively.Compared with that before the establishment of the model,the proportion of burst firing neurons in the right VM was significantly higher after the establishment of the model(P=0.001).Compared with that in the left VM,the proportion of burst firing neurons in the right VM increased significantly in the model rats and the difference was statistically significant(χ^(2)=8.355,P=0.004).Conclusion In rats with unilateral 6-OHDA lesions,the firing activity of the ventromedial thalamic nucleus changed significantly.The ventromedial thalamic nucleus may be important in the pathophysiology of Parkinson’s disease.

关 键 词：帕金森病 丘脑腹内侧核 电生理 6-羟基多巴胺 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]