安罗替尼联合放射治疗对食管鳞状细胞癌细胞增殖、凋亡及放射治疗敏感性的影响  

Effect of anlotinib combined with radiotherapy on the proliferation,apoptosis and radiotherapy sensitivity of esophageal squamous cell carcinoma cells

作　　者：董起杭 万里新[2] 张凯 王卓 陶海云[2] 杜云辉 高社干[5,6] 兰子君 原翔 DONG Qihang;WAN Lixin;ZHANG Kai;WANG Zhuo;TAO Haiyun;DU Yunhui;GAO Shegan;LAN Zijun;YUAN Xiang(Xinxiang Medical University,Xinxiang 453003,Henan Province,China;Department of Oncology,Nanyang Central Hospital,Nanyang 473000,Henan Province,China;Department of Radiotherapy,Nanyang Central Hospital,Nanyang 473000,Henan Province,China;Department of Pharmacy,Nanyang Central Hospital,Nanyang 473000,Henan Province,China;Department of Oncology,the First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471003,Henan Province,China;Henan Provincial Key Laboratory of Tumor Epigenetics,Luoyang 471003,Henan Province,China)

机构地区：[1]新乡医学院,河南新乡453003 [2]南阳市中心医院肿瘤内科,河南南阳473000 [3]南阳市中心医院放射治疗科,河南南阳473000 [4]南阳市中心医院药学科,河南南阳473000 [5]河南科技大学第一附属医院肿瘤内科,河南洛阳471003 [6]河南省肿瘤表观遗传学重点实验室,河南洛阳471003

出　　处：《新乡医学院学报》2022年第2期106-112,共7页Journal of Xinxiang Medical University

摘　　要：目的探讨安罗替尼联合放射治疗对食管鳞状细胞癌细胞的增殖、凋亡及放射治疗敏感性的影响。方法将对数生长期的食管鳞状细胞癌KYSE-150细胞随机分为0μmol·L^(-1)安罗替尼组、2μmol·L^(-1)安罗替尼组、4μmol·L^(-1)安罗替尼组、8μmol·L^(-1)安罗替尼组、16μmol·L^(-1)安罗替尼组、32μmol·L^(-1)安罗替尼组、64μmol·L^(-1)安罗替尼组、128μmol·L^(-1)安罗替尼组,分别用含终浓度为0、2、4、8、16、32、64、128μmol·L^(-1)安罗替尼的培养基进行培养。采用细胞计数试剂盒-8法检测8组食管鳞状细胞癌KYSE-150细胞的增殖能力,并计算细胞增殖抑制率。应用Graphpad Prism8软件绘制细胞增殖抑制曲线并计算半抑制浓度(IC 50),选择IC 50值最小时的安罗替尼浓度及处理时间作为后续实验的药物处理条件。另收集对数生长期的食管鳞状细胞癌KYSE-150细胞,随机分为0 Gy单独照射组、2 Gy单独照射组、4 Gy单独照射组、6 Gy单独照射组、0 Gy联合照射组、2 Gy联合照射组、4 Gy联合照射组、6 Gy联合照射组。0 Gy单独照射组、2 Gy单独照射组、4 Gy单独照射组、6 Gy单独照射组细胞分别用不含安罗替尼的培养液培养48 h后,更换不含安罗替尼的培养液,并分别给予0、2、4、6 Gy的X射线照射;0 Gy联合照射组、2 Gy联合照射组、4 Gy联合照射组、6 Gy联合照射组使用含终浓度4μmol·L^(-1)安罗替尼的培养液培养48 h后,更换为不含安罗替尼的培养液,同时分别给予0、2、4、6 Gy的X射线照射。采用平板克隆形成实验检测不同X射线照射剂量的单独照射组和联合照射组细胞的克隆形成能力,并计算各组的存活率。将0 Gy单独照射组、2 Gy单独照射组、4 Gy单独照射组、6 Gy单独照射组归为单独照射组,将0 Gy联合照射组、2 Gy联合照射组、4 Gy联合照射组、6 Gy联合照射组细胞归为联合照射组,采用单击多靶模型检测安罗替尼联合放射Objective To explore the effects of anlotinib combined with radiotherapy on the proliferation,apoptosis and radiotherapy sensitivity of esophageal squamous cell carcinoma cells.Methods The esophageal squamous cell carcinoma KYSE-150 cells in the logarithmic growth phase were randomly divided into 0μmol·L^(-1) anlotinib group,2μmol·L^(-1) anlotinib group,4μmol·L^(-1) anlotinib group,8μmol·L^(-1) anlotinib group,16μmol·L^(-1) anlotinib group,32μmol·L^(-1) anlotinib group,64μmol·L^(-1) anlotinib group,128μmol·L^(-1) anlotinib group,and cultured with culture medium containing final concentrations of 0,2,4,8,16,32,64,128μmol·L^(-1) anlotinib,respectively.The proliferation ability of esophageal squamous cell carcinoma KYSE-150 cells in the eight groups was detected by the cell counting kit-8 method,and the cell proliferation inhibition rate was calculated.The cell proliferation inhibition curve was drawed by Graphpad Prism 8 software and the half-inhibitory concentration(IC 50)was obtained.The concentration and treatment time of anlotinib at the minimum IC 50 value were selected as the drug treatment conditions for the subsequent experiments.In addition,the esophageal squamous cell carcinoma KYSE-150 cells in the logarithmic growth phase were collected and randomly divided into 0 Gy single irradiation group,2 Gy single irradiation group,4 Gy single irradiation group,6 Gy single irradiation group,0 Gy combined irradiation group,2 Gy combined irradiation group,4 Gy combined irradiation group,6 Gy combined irradiation group.The cells in the 0 Gy single irradiation group,2 Gy single irradiation group,4 Gy single irradiation group and 6 Gy single irradiation group were cultured with anlotinib-free medium for 48 hours,and then replaced with anlotinib-free medium,and X-ray irradiation of 0,2,4,6 Gy was given,respectively;the cells in the 0 Gy combined irradiation group,2 Gy combined irradiation group,4 Gy combined irradiation group and 6 Gy combined irradiation group were cultured for 48 hours with culture m

关 键 词：安罗替尼 食管鳞状细胞癌 放射治疗敏感性 

分 类 号：R735.1[医药卫生—肿瘤]