A reversible microarray immobilization strategy based on thiol-quinone reaction  

作　　者：Ling Feng Ping Wang Yi Feng Jie Zhang Qingxin Chen Yusheng Xie Jingdong Luo Jiang Xia Shao Q.Yao Hongyan Sun 

机构地区：[1]Cancer and Aging Research Institution,School of Life Science,Shandong University of Technology,Zibo 255000,China [2]Department of Chemistry and COSDAF(Centre of Super-Diamond and Advanced Films),City University of Hong Kong,Hong Kong,China [3]Key Laboratory of Biochip Technology,Biotech and Health Centre,Shenzhen Research Institute of City University of Hong Kong,Shenzhen 518057,China [4]College of Food Engineering,Qingdao Institute of Technology,Qingdao 266300,China [5]Department of Chemistry,The Chinese University of Hong Kong,Hong Kong,China [6]Department of Chemistry,National University of Singapore,Singapore 117543,Singapore

出　　处：《Chinese Chemical Letters》2022年第1期213-216,共4页中国化学快报（英文版）

基　　金：the financial support from the Research Grants Council of Hong Kong(Nos.11102719,11304118 and 14306317);Shenzhen Basic Research Project(No.JCYJ20160601173218804);National Natural Science Foundation of China(No.21778044);the Shandong University of Technology Ph.D.Startup Foundation(No.420033)。

摘　　要：Microarray technology has been widely applied in biomedical research.The key to microarray study is to develop efficient immobilization method.In this study,we designed a new reversible microarray immobilization method based on thiol-quinone reaction.A quinone-functionalized slide was fabricated through H_(2)O_(2)treatment of dopamine-coated slides.Various thiol-containing molecules can be anchored onto the quinone-functionalized slides via thioether linker,which could be cleaved under H_(2)O_(2) treatment to regenerate quinone groups on the surface.The highly versatile approach can be widely used for immobilization of various thiol-containing molecules.

关 键 词：Reversible Microarray BIOCHIP Thiol-quinone reaction Peptides Thiol-proteins 

分 类 号：R318[医药卫生—生物医学工程] O621.25[医药卫生—基础医学]