Profiling of seminal antioxidant indices and sperm quality in Plasmodium bergheiinduced malarial mice treated with Phyllanthus amarus  

作　　者：Williams Oshiegbu Chinwendu Obogheneophruhe Elu Innocent Onyesom 

机构地区：[1]Department of Biochemistry,Delta State University,Abraka,Nigeria [2]Department of Medical Biochemistry,Delta State University,Abraka,Nigeria

出　　处：《Asian pacific Journal of Reproduction》2022年第2期84-92,共9页亚太生殖杂志（英文版）

摘　　要：Objective:To evaluate the antiplasmodial activity of Phyllanthus(P.)amarus crude ethanol leaf extract and its effects on semen quality in male BALB/c mice.Methods:A total of 36 adult mice were divided into six groups,with 6 mice each.Five groups were infected with Plasmodium(P.)berghei,and one group was left uninfeceted.Of the five infected groups,one group was left untreated,three groups were treated with varying doses(100,250 and 400 mg/kg)of P.amarus crude ethanol leaf extract orally for 4 days,and another group was treated with standard drug,artemether and lumefantrine(Lonart®DS).Antiplasmodial activity,seminal quality,some biochemical indices(neutral毩-glucosidase,fructose,and citric acid)in seminal plasma and seminal antioxidant markers(catalase,glutathione peroxidase,reduced glutathione,malondialdehyde,total antioxidant capacity,and acid phosphates)were determined.The mice were euthanized 3 days post treatment and semen was collected from the caudal epididymis and processed for analysis using documented methods and procedures.Results:Malarial infection led to oxidative stress,causing a significant decline in seminal quality(P<0.05).However,treatment with P.amarus crude ethanol leaf extract alleviated oxidative stress and significantly improved seminal quality.The improvement was dose-dependent and compared well with the standard drug,artemether and lumefantrine(Lonart®DS)treatment.Conclusions:The ethanol leaf extracts of P.amarus alleviate male reproductive capacity during malaria infection in murine model by enhancing antioxidant activities.

关 键 词：MALARIA Sperm quality Phyllanthus amarus Seminal oxidative stress Seminal antioxidant markers ANTIOXIDANTS Ethanol extracts ANTIMALARIALS 

分 类 号：R28[医药卫生—中药学]