骨髓间充质干细胞在缺血性脑卒中的治疗作用  

The therapeutic effect of bone marrow mesenchymal stem cells in ischemic stroke

作　　者：付丽环 江小霞[2] 毛更生[1] Fu Lihuan;Jiang Xiaoxia;Mao Gengsheng(Clinical College of Anhui Medical University,Hefei 230032;Institute of Military Cognition and Brain Sciences,Academy of Military Medical Sciences,Beijing 100850)

机构地区：[1]安徽医科大学临床学院,合肥230032 [2]军事医学科学院军事认知与脑科学研究所,北京100850

出　　处：《安徽医科大学学报》2022年第3期337-344,共8页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:31971285);北京市自然科学基金(编号:7202149)。

摘　　要：目的探讨骨髓间充质干细胞(BMSCs)对大鼠缺血性脑卒中的疗效和作用机制。方法采用全骨髓贴壁法分离BMSCs,用流式细胞术鉴定BMSCs;制备大脑中动脉闭塞(MCAO)大鼠模型,随机分为假手术组、MCAO组和BMSC组;对各组大鼠进行mNSS评分和TTC染色,并采用免疫组织化学法、免疫荧光法和免疫印迹法检测各组脑组织梗死区及其周围关于Caspase-3、MAP-2、GAP-43及GFAP的阳性表达水平。结果BMSCs低表达CD45,高表达CD90、CD29;大鼠在移植BMSCs后第3、7和14天的mNSS评分和梗死体积百分比均降低,其梗死区的Caspase-3下降,MAP-2、GFAP和GAP-43表达增强。结论BMSCs移植能够促进MCAO模型大鼠的神经功能恢复和减小脑梗死体积,可能是通过抑制细胞凋亡、轴突再生、神经再生细胞增殖等机制发挥其疗效。Objective To explore the therapeutic effect and mechanism of bone marrow mesenchymal stem cells(BMSCs)on ischemic stroke in rats.Methods BMSCs were isolated by whole bone marrow adherence method and identified by flow cytometry.The rat model of middle cerebral artery occlusion(MCAO)was established and randomly divided into sham operation group,MCAO group and BMSC group.The rats in each group were scored by mNSS and stained with TTC.Immunohistochemistry,immunofluorescence and Western blot were used to detect the positive expression levels of Caspase-3,MAP-2,GAP-43 and GFAP in the infarct area and surrounding areas of the brain tissues of each group.Results Flow cytometry detected that BMSCs expressed low CD45 and high expression of CD90 and CD29;The mNSS score and the percentage of infarct volume of rats on the 3rd,7th and 14th day after transplantation of BMSCs all decreased;The Caspase-3 in the infarct area decreased,and the expression of MAP-2,GFAP and GAP-43 increased.Conclusion BMSCs transplantation can promote the recovery of neurological function and reduce the volume of cerebral infarction in MCAO model rats,possibly through mechanisms such as nerve regeneration cell proliferation,inhibition of cell apoptosis,and axon regeneration.

关 键 词：骨髓间充质干细胞 缺血性脑卒中 尾静脉 神经修复 

分 类 号：R743.3[医药卫生—神经病学与精神病学]