CCL20通过ERK通路促进食管鳞癌细胞迁移和侵袭  

CCL20 promotes esophageal squamous cell carcinoma cells migration and invasion through ERK pathway

作　　者：南红星 禹刘丽 王良海[1] Nan Hongxing;Yu Liuli;Wang Lianghai(Dept of Pathology,The First Affiliated Hospital of Shihezi University School of Medicine,Shihezi 832000;Dept of Pathology and Key Laboratory of Xinjiang Endemic and EthnicDiseases,Shihezi University School of Medicine,Shihezi 832000)

机构地区：[1]石河子大学医学院第一附属医院病理科,石河子832000 [2]新疆地方病与民族病病理学重点实验室,石河子832000

出　　处：《安徽医科大学学报》2022年第3期396-401,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:82060513、81902850)。

摘　　要：目的研究趋化因子配体20(CCL20)对食管鳞癌细胞迁移、侵袭和增殖的影响,并初步探讨其分子机制。方法转染CCL20表达质粒和干扰质粒至食管鳞癌细胞,用G418筛选稳定转染细胞,Western blot或实时定量PCR实验评估过表达和干扰效果。采用Transwell和CCK8实验检测CCL20对食管鳞癌细胞迁移、侵袭和增殖的影响,用通路抑制剂寻找CCL20的下游通路。结果成功建立CCL20过表达细胞株和干扰细胞株,过表达CCL20可促进食管鳞癌细胞迁移和侵袭,同时增加ERK1/2蛋白的磷酸化激活。干扰CCL20可降低食管鳞癌细胞的迁移侵袭能力,减少ERK1/2蛋白磷酸化激活。另外,ERK1/2特异性抑制剂可以逆转过表达CCL20导致的迁移和侵袭。结论CCL20通过激活ERK通路促进食管鳞癌细胞迁移和侵袭,CCL20可能成为食管鳞癌治疗的潜在靶点。Objective To investigate the influence of C-C motif chemokine ligand 20(CCL20)on migration,invasion and proliferation of esophageal squamous cell carcinoma(ESCC)cells,and to preliminarily research its molecular mechanism.Methods The CCL20 expression plasmid and interference plasmid was transfected into ESCC cells,and the stably transfected cells was selected with G418,Western blot or qRT-PCR assay was used to assess the effects of over-expression and interference.The influence of CCL20 in migration,invasion and proliferation of ESCC cells was detected by Transwell and CCK8 assays,pathway inhibitors was used to seek the downstream signaling pathway of CCL20.Results CCL20 overexpressing and interfering cell lines was successfully established,CCL20 overexpression promoted ESCC cells migration and invasion,and increased the activation level of ERK1/2.Interfering CCL20 expression reduced the ability of migration and invasion in ESCC cells,and the activation level of ERK1/2 decreased.Moreover,ERK1/2 specific inhibitor treatment reversed the migration and invasion induced by CCL20.Conclusion CCL20 promotes ESCC cells migration and invasion by activating ERK pathway.CCL20 may be a potential target for the treatment of ESCC.

关 键 词：食管鳞状细胞癌 CCL20 转移 迁移 侵袭 

分 类 号：R735.1[医药卫生—肿瘤]