circFAT1在非小细胞肺癌组织中表达及对细胞增殖和侵袭力的影响  

Expression of circFAT1 in non-small cell lung cancer tissues and its effect on cell proliferation and invasiveness

作　　者：徐至珺 王炯[1] 解明然 颜宇 张倩[1] 周文勤[1] Xu Zhijun;Wang Jiong;Xie Mingran;Yan Yu;Zhang Qian;Zhou Wenqin(Dept of Geriatric Respiratory and Critical Care Medicine,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;Dept of Thoracic Surgery,The First Affiliated Hospital of University of Science and Technology of China,Hefei 230001;Dept of Respiratory Medicine,Anhui Children′s Hospital,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院老年呼吸与危重症医学科,合肥230022 [2]中国科学技术大学附属第一医院胸外科,合肥230001 [3]安徽省儿童医院呼吸科,合肥230022

出　　处：《安徽医科大学学报》2022年第3期464-468,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81973643);安徽省重点研究与开发计划项目(编号:006143314006)。

摘　　要：目的探讨circFAT1在非小细胞肺癌(NSCLC)组织中表达情况及沉默circFAT1对NSCLC细胞A549增殖和侵袭力的影响。方法选取行手术切除治疗的NSCLC患者107例,RT-qPCR检测NSCLC和癌旁组织中circFAT1表达,培养A549细胞,分别转染siRNA-circFAT1(si-circFAT1组)、无关序列(si-control组),另外设未转染细胞作为空白组(blank组),RT-qPCR检测细胞中circFAT1表达量,CCK-8检测细胞增殖情况,Transwell小室检测细胞迁移和侵袭情况。结果NSCLC组织中circFAT1相对表达量高于癌旁组织[(2.47±0.27)vs(1.02±0.15),t=48.959,P<0.001];与TNM分期Ⅰ~Ⅱ期、中高分化、未发生淋巴结转移比较,TNM分期Ⅲ~Ⅳ期、低分化和发生淋巴结转移的NSCLC组织中circFAT1相对表达量升高(P<0.05);与si-control组和blank组比较,si-circFAT1组细胞中circFAT1相对表达量明显降低(P<0.05),迁移细胞数和侵袭细胞数减少(P<0.05)。结论NSCLC组织中circFAT1相对表达量升高,沉默circFAT1可抑制A549细胞的增殖、迁移和侵袭能力。Objective To investigate the expression of circFAT1 in non-small cell lung cancer(NSCLC)tissues and the effect of silencing circFAT1 on the proliferation and invasiveness of NSCLC cell A549.Methods A total of 107 patients with NSCLC who underwent surgical resection were selected.Real-time fluorescent quantitative PCR(RT-qPCR)was used to detect the expressions of circFAT1 in NSCLC and adjacent tissues.A549 cells were cultured and transfected with siRNA-circFAT1(si-circFAT1 group)and irrelevant sequences(si-control group),respectively,and in addition,the untransfected cells were set as the blank group.RT-qPCR was used to detect the expression of circFAT1 in cells,CCK-8 was used to detect cell proliferation,and Transwell chamber was used to detect cell migration and invasion.Results The relative expression level of circFAT1 in NSCLC tissues was higher than that in the adjacent tissues[(2.47±0.27)vs(1.02±0.15),t=48.959,P<0.001].Compared with TNM stagesⅠ-Ⅱ,moderately well differentiated and no lymph node metastasis,the relative expression levels of circFAT1 in NSCLC tissues with TNM stagesⅢ-Ⅳ,poorly differentiated and lymph node metastasis increased(P<0.05).Compared with the si-control group and the blank group,the relative expression level of circFAT1 in the cells of the si-circFAT1 group was significantly reduced(P<0.05),and the numbers of migrating cells and invading cells were reduced(P<0.05).Conclusion The relative expression level of circFAT1 in NSCLC tissues increased.Silencing circFAT1 can inhibit the proliferation,migration and invasion of A549 cells.

关 键 词：非小细胞肺癌 circFAT1 临床指标 细胞增殖 细胞侵袭 

分 类 号：R734.2[医药卫生—肿瘤]