血浆proGRP和I-TAC蛋白在小细胞肺癌中的意义  

Potential Value of Plasma proGRP and I-TAC Proteins in Small Cell Lung Cancer

作　　者：刘丹[1] 韩贵良[1] 霍悦 贾友超[1] 李玉苗 赵琳珊 高翔鹏 赵国发 王晓芳 臧爱民[1] LIU Dan;HAN Gui-liang;HUO Yue;JIA You-chao;LI Yu-miao;ZHAO Lin-shan;GAO Xiang-peng;ZHAO Guo-fa;WANG Xiao-fang;ZANG Ai-min(Affiliated Hospital of Hebei University,Hebei Key Laboratory of Cancer Radiotherapy and Chemotherapy,Banding 071000,China)

机构地区：[1]河北大学附属医院,河北省肿瘤放化疗机制与规程研究重点实验室,河北保定071000

出　　处：《肿瘤学杂志》2022年第1期19-27,共9页Journal of Chinese Oncology

基　　金：河北省政府资助临床医学优秀人才课题(361007);河北大学附属医院青年科研基金项目(2017Q021;2019Q004)。

摘　　要：[目的]分析血浆样本中的差异蛋白在小细胞肺癌(small cell lung cancer,SCLC)诊断及预后中的价值。[方法]首先应用GSH-CAA-X00蛋白芯片技术检测4例SCLC治疗前后及4名健康人外周血,结合生物信息学分析筛选差异蛋白。应用ELISA检测36例SCLC治疗前后及8名健康人血浆样本中关键差异蛋白胃泌素释放肽前体(progastrin releasing peptide,proGRP)、干扰素诱导T细胞趋化因子(interferon-inducible T cell alphachemoattractant,I-TAC)的表达,分析其表达与SCLC临床指征之间的关系。[结果]蛋白芯片结果显示在健康人和SCLC患者治疗前血浆样本中符合筛选且有统计学意义的差异蛋白有PDGF-AB、S100A8、GHR、GP73、GRO、proGRP、I-TAC等,在SCLC患者治疗前后血浆样本中符合筛选且有统计学意义的差异蛋白有MMP-3、NSE、LRIG3、proGRP、ITAC等。PDGF-AB、S100A8、GHR、GP73、GRO、MMP-3、NSE、proGRP、I-TAC在健康人、SCLC患者治疗前、治疗后的表达趋势为先上升后下降,其中proGRP、I-TAC在两组间的变化最为显著且都具有统计学意义。ELISA结果显示proGRP、I-TAC与芯片结果一致,即与健康人相比,在SCLC患者表达升高,经治疗后下降,但与临床指征无相关性。[结论]差异蛋白尤其是proGRP、I-TAC可能是SCLC的潜在诊治肿瘤标志物,单独使用或与其他常用生物标志物联合使用可能有助于SCLC的早期诊断及预后判断。[Objective]To investigate plasma proteins with potential value for diagnosis and prognosis of small cell lung cancer(SCLC).[Methods]The differentially expressed proteins were detected with GSH-CAA-X00 protein chip and analyzed with bioinformatics from plasma samples of 4 cases of SCLC before and after treatment,and 4 healthy subjects.The key differential proteins progastrin releasing peptide(proGRP)and interferon-inducible T cell alpha chemoattractant(I-TAC)were detected with ELISA in plasma samples of 36 SCLC patients before and after treatment and 8 healthy subjects.[Results]The proteins differentially expressed between SCLC patients before treatment and healthy subjects were PDGF-AB,S100A8,GHR,GP73,GRO,proGRP,I-TAC,etc.The proteins differentially expressed in SCLC patients before and after treatment were MMP-3,NSE,LRIG3,I-TAC,etc.Compared with healthy controls,the plasma levels of PDGF-AB,S100A8,GHR,GP73,GRO,MMP-3,NSE,LRIG3,proGRP,ITAC in SCLC patients were significantly increased,then decreased after treatment,among which the changes of proGRP and I-TAC were the most significant.ELISA results showed that the changes of proGRP and I-TAC were consistent with the chip results,however,which were not correlated with clinical indications.[Conclusion]Differentially expressed proteins in plasma,especially proGRP and I-TAC,may be used as potential tumor markers for diagnosis and prognosis of SCLC.

关 键 词：小细胞肺癌 肿瘤标志物 胃泌素释放肽前体 干扰素诱导T细胞趋化因子 

分 类 号：R734.2[医药卫生—肿瘤]