基于人工神经网络算法的2株木质素降解菌固体菌剂的制备  

Preparation of two strains of lignin-degrading bacteria solid inoculum

作　　者：李雅琳 李素艳[1] 孙向阳[1] 郝丹 蔡琳琳 常晓彤 LI Yalin;LI Suyan;SUN Xiangyang;HAO Dan;CAI Linlin;CHANG Xiaotong(College of Forestry,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学林学院,北京100083

出　　处：《浙江农林大学学报》2022年第2期364-371,共8页Journal of Zhejiang A&F University

基　　金：北京市自然科学基金资助项目(6202021)。

摘　　要：【目的】以2株不同的木质素降解菌[菌株A(构巢曲霉Aspergillus nidulans)、菌株Q(栓菌属1种Trametes sp.)]为材料,分别制作应用于园林绿化废弃物降解或者堆肥的高效固体发酵菌剂。【方法】采用单因素试验确定固体发酵培养基的碳氮源和外加营养组分种类,再通过正交试验对碳氮源添加量进行优化,最后根据碳氮源优化结果,采用均匀实验结合人工神经网络算法寻找2株木质素降解菌的外加营养组分接种量和最佳固体培养基发酵条件。【结果】优化后的菌株Q固体菌剂培养基基质以麸皮30.000 g作为基底,添加豆饼粉3.000 g和玉米粉0.188 g;外加营养组分(按基质的质量比)为硫酸镁(MgSO_(4))1.434%、磷酸二氢钾(KH_(2)PO_(4))0.115%和硫酸亚铁(FeSO_(4)·7H_(2)O)1.497%;接种条件为接菌量6.000%、料水比(质量比)1.000∶0.992、保护剂1.000%。优化后的菌株A固体菌剂培养基基质以麸皮30.000 g作为基底,添加豆饼粉1.500 g和木质素磺酸钠1.500 g;外加营养组分(按基质的质量比)为MgSO_(4)0.123%、KH_(2)PO_(4)0.213%、FeSO_(4)·7H_(2)O 1.280%;接种条件为接菌量21.000%、料水比1∶1、保护剂19.000%。菌株Q和菌株A在优化培养基上发酵7~9 d获得最大发酵生物量,菌株Q的吸光度D(260)为0.596,菌株A的D(260)为0.478。【结论】2株木质素降解菌在优化后的发酵条件下制得的固体菌剂具有高生物量的特点,在降解园林绿化废弃物的木质素方面具有一定潜力。[Objective]With two different lignin-degrading bacteria,strain A(Aspergillus nidulans)and strain Q(Trametes sp.)used as materials,this study is aimed to research the production of high-efficiency solid fermentation inoculants for degradation or composting of garden greening wastes.[Method]First,a single factor experiment was employed to determine the types of carbon and nitrogen sources and additional nutrient components of the solid fermentation medium.Then,the orthogonal experiment design was adopted to optimize the amount of carbon and nitrogen sources to be added,based on which,efforts were made to figure out the inoculation amount of additional nutrient components and the best solid medium fermentation conditions of the two lignin-degrading bacteria using a uniform experiment combined with the artificial neural network algorithm.[Result]The optimized solid inoculum medium matrix(mass ratio)of strain Q was with 30.000 g bran as the base,3.000 g soybean meal powder and 0.188 g corn flour added,with extra nutrients(based on the mass ratio of the matrix)being MgSO_(4)1.434%,KH_(2)PO_(4)0.115%,FeSO_(4)·7H_(2)O 1.497%whereas the inoculation conditions being 6.000%of inoculation,with the ratio of material to water as 1.000∶0.992,and the protective agent as 1.000%.The optimized solid inoculum medium matrix(mass ratio)of strain A was with 30.000 g bran as the base,1.500 g soybean meal powder and 1.500 g sodium lignosulfonate were added,with extra nutrients(based on the mass ratio of the matrix)being MgSO_(4)0.123%,KH_(2)PO_(4)0.213%,FeSO_(4)·7H_(2)O 1.280%whereas the inoculation conditions being 21.000%inoculation amount with the ratio of material to water as 1∶1 and the protective agent as 19.000%.Strain Q and strain A were fermented on the optimized medium for 7−9 days to get the largest fermentation biomass with the D(260)value of strain Q being 0.596 and the D(260)value of strain A being 0.478.[Conclusion]The solid inoculum prepared by two lignin-degrading bacteria under optimized fermentation conditions

关 键 词：木质素降解菌 优化 固体菌剂 发酵 

分 类 号：S142[农业科学—肥料学]