基于UPLC-MS/MS大蒜肠溶制剂中蒜氨酸、蒜酶体内反应情况研究  

Study on the Reaction of Garlic and Uterine in the UPLC-MS/MS of Garlic Sausol

作　　者：杨亮 胡小霞 宋百灵 关明[3] 李新霞[2] YANG Liang;HU Xiaoxia;SONG Bailing;GUAN Ming;LI Xinxia(Xinjiang Police College,Urumqi 830054,Xinjiang China;Chemistry and Chemical Engineering of Xinjiang Normal University College,Urumqi 830054,Xinjiang China;School of Pharmacy,Xinjiang Medical University,Urumqi 830011,Xinjiang China;Central Laboratory of Xinjiang Medical University,Urumqi 830011,Xinjiang China)

机构地区：[1]新疆警察学院,新疆乌鲁木齐830011 [2]新疆医科大学药学院,新疆乌鲁木齐830011 [3]新疆师范大学化学化工学院,新疆乌鲁木齐830054 [4]新疆医科大学中心实验室新,疆乌鲁木齐830011

出　　处：《生命科学仪器》2021年第6期66-71,共6页Life Science Instruments

基　　金：国家自然科学基金资助项目(No.81560564,21465023)。

摘　　要：目的建立定量测定动物血浆中蒜氨酸含量的UPLC-MS/MS方法,研究大蒜肠溶制剂中蒜氨酸、蒜酶能否在体内环境下反应生成活性成分大蒜辣素。方法采用反相C18色谱柱(Waters ICQUITY UPLCBEH,100×2.1 mm,1.7μm),柱温:40℃,流速:0.15 mL/min,进样量:2μl,流动相:0.1%甲酸(A)-乙腈(B),梯度洗脱,质谱离子化方式:ESI+,测定大鼠血浆中蒜氨酸的含量。结果大蒜肠溶制剂和灭酶蒜粉两组平行实验的结果表明,在有蒜酶存在的大蒜肠溶制剂中,大鼠血液中蒜氨酸血药浓度明显偏低。结论建立了定量测定动物血浆中蒜氨酸的UPLC-MS/MS方法,大蒜肠溶制剂中蒜氨酸和蒜酶能够在体内环境下发生反应。A UPLC-MS/MS method was established to quantitatively determine the content of alliin in animal plasma to study whether alliin and alliin in garlic enteric preparations can react to produce the active ingredient allicin in the in vivo environment. Methods Reversed-phase C18 column(Waters ICQUITY UPLC BEH, 100 ×2.1 mm, 1.7μm), column temperature: 40 ℃, flow rate: 0.15 mL/min, injection volume: 2μl, Mobile phase: 0.1%formic acid(A)-acetonitrile(B), gradient elution;mass spectrometry ionization: ESI+, determination of allicin in rat plasma. Results The results of two parallel experiments of garlic enteric preparation and enzymatic garlic powder showed that in the garlic enteric preparation with allinase, the plasma concentration of alliin in the blood of rats was significantly lower. Conclusion A UPLC-MS/MS method for the quantitative determination of alliin in animal plasma has been established. Alliin and alliin in garlic enteric-coated preparations can react in vivo.

关 键 词：肠道反应 蒜氨酸 超高效液相色谱质谱联用 

分 类 号：O657.6[理学—分析化学]