Kiwifruit(Actinidia chinensis 'Hongyang') cytosolic ascorbate peroxidases(AcAPX1 and AcAPX2) enhance salinity tolerance in Arabidopsis thaliana  

作　　者：GUO Xiu-hong HE Yan ZHANG Yu WANG Yi HUANG Sheng-xiong LIU Yong-sheng LI Wei 

机构地区：[1]Key Laboratory for Bio-resource and Eco-environment of Ministry of Education,College of Life Sciences/State Key Laboratory of Hydraulics and Mountain River Engineering,Sichuan University,Chengdu 610064,P.R.China [2]School of Horticulture,Anhui Agricultural University,Hefei 230036,P.R.China [3]School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第4期1058-1070,共13页农业科学学报（英文版）

基　　金：funded by the National Natural Science Foundation of China (31972474);the Natural Science Research Program of Universities of Anhui Province, China (K1832004);the Leading Talent Group Funding of Anhui Province, China (WRMR-2020-75);the Natural Science Foundation of Anhui Province, China (19232002);the Anhui Agriculture University Shennong Scholar Project, China (RC321901)。

摘　　要：Ascorbate peroxidase(APX) plays a key role in scavenging reactive oxygen species(ROS) in higher plants. However, there is very little information available on the APXs in kiwifruit(Actinidia), which is an economically and nutritionally important horticultural crop with exceptionally high ascorbic acid(AsA) accumulation. This study aims to identify and characterize two cytosolic APX genes(AcAPX1 and AcAPX2) derived from A. chinensis ‘Hongyang’. The constitutive expression pattern was determined for both AcAPX1 and AcAPX2, and showed relatively higher expression abundances of AcAPX1 in leaf and AcAPX2 in root. Transcript levels of AcAPX1 and AcAPX2 were increased in kiwifruit roots treated with Na Cl. Subcellular localization assays using GFP-fusion proteins in Arabidopsis protoplasts showed that both AcAPX1 and AcAPX2 are targeted to the cytosol. Recombinant AcAPX1 or AcAPX2 proteins were successfully expressed in the prokaryotic expression system and their individual ascorbate peroxidase activities were determined. Finally, constitutive over-expression of AcAPX1 or AcAPX2 could dramatically increase total As A, glutathione level and salinity tolerance under Na Cl stress in Arabidopsis thaliana. Our findings revealed that cytosolic AcAPX1/2 may play an important protective role in the responses to unfavorable environmental stimuli in kiwifruit.

关 键 词：ACTINIDIA ascorbic acid ascorbate peroxidase SALINITY 

分 类 号：S663.4[农业科学—果树学]