The new effector AbSCP1 of foliar nematode(Aphelenchoides besseyi) is required for parasitism rice  

作　　者：HUANG Xin CHI Yuan-kai Addisie Abate BIRHAN ZHAO Wei QI Ren-de PENG De-liang 

机构地区：[1]Institute of Plant Protection and Agro-products Safety,Anhui Academy of Agricultural Sciences,Hefei 230001,P.R.China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China [3]Agricultural Biotechnology Directorate,Ethiopian Biotechnology Institute,Addis Ababa 5954,Ethiopia

出　　处：《Journal of Integrative Agriculture》2022年第4期1084-1093,共10页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31701774);the Natural Science Foundation of Anhui Province,China(2008085QC123)。

摘　　要：Plant parasitic nematodes secrete effector proteins to parasitize hosts successfully. Of these proteins, serine carboxypeptidases have critical roles in pathogenicity. This study investigated the role of new effector AbSCP1 in Aphelenchoides besseyi pathogenicity. In situ hybridization and qRT-PCR analyses indicated that AbSCP1 was exclusively expressed in the esophageal glands and upregulated in juveniles. Subcellular localization assays indicated that the protein was expressed in the nucleus. The ability to hydrolyze C-terminal amino acid residues was proven for AbSCP1. Moreover, RNAi significantly reduced the expression of AbSCP1 and RNAi-treated nematodes’ reproductive potential. Pathogenicity assays on rice showed that RNAi-treated nematodes were less pathogenic than the untreated control groups. These results suggest the important role of AbSCP1 in the A. besseyi infection process.

关 键 词：Aphelenchoides besseyi serine carboxypeptidases EFFECTOR NEMATODE PATHOGENICITY 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]