miR-99a-5p inhibits target gene FZD5 expression and steroid hormone secretion from goat ovarian granulosa cells  

作　　者：ZHU Lu JING Jing QIN Shuai-qi LU Jia-ni ZHU Cui-yun ZHENG Qi LIU Ya FANG Fu-gui LI Yun-sheng ZHANG Yun-hai LING Ying-hui 

机构地区：[1]College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,P.R.China [2]Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province,Hefei 230036,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第4期1137-1145,共9页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31772566 and 31972629);the Central Guidance on Local Science and Technology Development Fund of Anhui Province,China(202007d06020005)。

摘　　要：MicroRNA(miRNA) has vital regulatory effects on the proliferation, differentiation and secretion of ovarian granulosa cells, but the role of miR-99a-5p in goat ovarian granulosa cells(GCs) is unclear. Both miR-99a-5p and Frizzled-5(FZD5) were found to be expressed in GCs in goat ovaries via fluorescence in situ hybridization and immunohistochemistry, respectively, and FZD5 was verified(P<0.001) as a target gene of miR-99a-5p by double luciferase reporter gene experiments. Furthermore, FZD5 mRNA and protein expression were both found to be regulated(P<0.05) by miR-99a-5p in GCs. Moreover, the overexpression of miR-99a-5p or knockdown of FZD5 suppressed(P<0.05) estradiol and progesterone secretion from the GCs, as determined by ELISA. In summary, miR-99a-5p inhibits target gene FZD5 expression and estradiol and progesterone synthesis in GCs. Our study thus provides seminal data and new insights into the regulatory mechanisms of follicular development in the goat and other animals.

关 键 词：miRNA Frizzled-5 follicular development ESTRADIOL PROGESTERONE 

分 类 号：S827[农业科学—畜牧学]