The protective effect of W026B on global cerebral ischemia/reperfusion injury model in rats  

W026B对大鼠全脑缺血再灌注损伤的保护作用

作　　者：Ziyi Wang Xiaoyan Liu Yuanjun Zhu Ye Liu Pingping Zhang Yinye Wang 王子翼;刘晓岩;朱元军;刘晔;张平平;王银叶(北京大学医学部药学院分子与细胞药理学系,北京100191;北京红惠新医药科技有限公司,北京102600)

机构地区：[1]Department of Molecular and Cellular Pharmacology,School of Pharmaceutical Sciences,Peking University Health Science Center,Beijing 100191,China [2]Beijing Honghui New Medical Technology Co.,Ltd.,Beijing Daxing Biological Medicine Industry Base,Beijing 102600,China

出　　处：《Journal of Chinese Pharmaceutical Sciences》2022年第2期108-116,共9页中国药学（英文版）

基　　金：National Natural Science Foundation of China(Grant No.81503060,81573333);R&D Foundation of Beijing Honghui New Medical Technology Co.,Ltd。

摘　　要：Cerebral ischemia seriously affects the quality of life and health of human worldwide.W026B is a newly synthesized lignan derivative that has a protective effect on the focal cerebral ischemia/reperfusion model,while it is unclear whether W026B has a cerebral protective effect on the model of global cerebral ischemia/reperfusion(GCI/R).In this study,we investigated the protective effect of W026B on the four-vessel occlusion GCI/R model.The results showed that W026B obviously increased the survival rate of rats during 7 d after GCI/R and significantly improved neurological deficits within 7 d after GCI/R.It evidently enhanced the number of survival neurons in the hippocampus of GCI/R rats.Furthermore,W026B notably lowered the level of ROS,and increased the activity of SOD in the hippocampus of GCI/R rats.Moreover,it also decreased the expression of NF-κB p65 and the level of IL-6 apparently.In addition,W026B evidently lowered the activity of caspase-3.In conclusion,this study firstly proves that W026B has the protective effect on GCI/R rats.Its cerebral protective effect maybe related to the inhibition of oxidative stress,inflammatory response,and cell apoptosis during GCI/R.These results provide new evidence with the protective effect of W026B on cerebral ischemia/reperfusion injury.脑缺血在世界范围内严重影响着人类的生活质量和生命健康。W026B是一种新合成的木脂素衍生物,对局灶性脑缺血/再灌注模型具有保护作用,但W026B对全脑缺血/再灌注(GCI/R)模型是否具有脑保护作用尚不清楚。本文研究了W026B对四血管闭塞性全脑缺血再灌注模型是否具有脑保护作用。结果显示:W026B明显提高了GCI/R后7天的大鼠存活率,显著改善了GCI/R后7天内的神经功能缺陷评分,明显增强了GCI/R大鼠海马中存活神经元的数量。此外,W026B明显降低了GCI/R大鼠海马组织中ROS的水平,并增加了SOD的活性;明显降低NF-κB p65的表达和IL-6的水平,W026B也降低了caspase-3的活性。这些结果首次证明了W026B对GCI/R大鼠具有脑保护作用,其作用可能与抑制GCI/R过程中的氧化应激、炎症反应和细胞凋亡有关。本研究为W026B对脑缺血/再灌注损伤的保护作用提供了新证据。

关 键 词：Global cerebral ischemia/reperfusion W026B NEUROBEHAVIOR Oxidative stress INFLAMMATION APOPTOSIS 

分 类 号：R962[医药卫生—药理学]