针刺对帕金森抑郁大鼠血清及脑组织SP含量的影响  

Effect of Acupuncture on SP Content in Serum and Brain Tissue of Rats with Parkinson’s Depression

作　　者：秦正巍 王顺[1] 白妍 李元 乔国强 安宁 付艺 QIN Zheng-wei;WANG Shun;BAI Yan;LI Yuan;QIAO Guo-qiang;AN Ning;FU Yi(Heilongjiang University of Traditional Chinese Medicine,Harbin Heilongjiang,150040,China;Heilongiiang Academy of Traditional Chinese Medicine,Harbin,Heilongjiang,150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江省中医药科学院,黑龙江哈尔滨150040

出　　处：《现代生物医学进展》2022年第3期423-426,417,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81674091)。

摘　　要：目的:探讨针刺(常规针刺、调神畅志针刺)对帕金森抑郁大鼠血清和脑中SP含量变化影响。方法:采取6-羟基多巴胺(6-OHDA)单侧纹状体双靶点注射法对大鼠进行帕金森(PD)造模并用阿普吗啡(APO)诱导实验检测。成功后,在PD模型基础上结合慢性不可预见温和应激(chronic unpredictable mildstress,CUMS)法进行帕金森抑郁(PDD)模型建立。将造模成功的40只PDD大鼠随机分为药物组、模型组、常规针刺组、调神畅志针刺组,每组10只,并设空白组10只,予相应治疗4周。采用免疫组织化学法和ELISA法对脑组织和血清中神经肽类P物质(SP)含量检测。结果:与空白组比较,模型组大鼠脑及血清SP含量显著升高(P<0.01)。与模型组比较,药物组大鼠脑及血清SP含量无明显变化(P>0.05),常规针刺组、调神畅志针刺组大鼠脑及血清SP含量均明显降低(P<0.05)。与常规针刺组相比,调神畅志针刺组大鼠脑及血清中SP显著降低(P<0.05,P<0.01)。结论:针刺对帕金森抑郁大鼠脑及血清中SP含量均有调节作用,且调神畅志针刺组优于常规针刺组,而美多芭与氟西汀药物对帕金森抑郁模型大鼠脑及血清中SP含量无明显调节作用。Objective:To investigate the effects of different acupuncture(ordinary acupuncture,Tiaoshen Changzhi acupuncture)on the changes of SP content in serum and brain tissue of rats with Parkinson’s depression.Methods:The model of Parkinson’s disease(PD)in rats was established by double target injection of 6-OHDA unilateral striatum and detected by APO induction test.After success,a PD model was established based on the chronic unpredictable mildstress(CUMS)method.Forty PDD model rats were randomly divided into drug group,model group,ordinary acupuncture group and Tiaoshen Changzhi acupuncture group,with 10 rats in each group and 10 rats in blank group.They were given corresponding drug therapy or acupuncture therapy for 4 weeks.Immunohistochemistry and ELISA were used to detect SP content in brain tissue and serum.Results:Compared with blank group,SP content in brain and serum of model group was significantly increased(P<0.01).Compared with the model group,the contents of SP in brain and serum of the drug group had no significant changes(P>0.05),but the contents of SP in brain and serum of the ordinary acupuncture group and the Tiaoshen Changzhi acupuncture group were significantly decreased(P<0.05).Compared with ordinary acupuncture group,SP in brain and serum of rats in Tiaoshen Changzhi acupuncture group was significantly decreased(P<0.05,P<0.01).Conclusion:Different acupuncture can regulate SP content in brain tissue and serum of rats with Parkinson’s depression,and Tiaoshen Changzhi acupuncture group is better than ordinary acupuncture group,while metobar and fluoxetine have no significant regulating effect on SP content in brain tissue and serum of rats with Parkinson’s depression.

关 键 词：常规针刺治疗 调神畅志针刺治疗 帕金森抑郁病 神经肽类P物质 

分 类 号：R-33[医药卫生] R742.5