青蒿琥酯对肺癌裸鼠新生血管生成的影响及机制相关研究  

Study on the Effect of Artesunate on Angiogenesis in Nude Mice with Lung Cancer and Its Mechanism

作　　者：陈耀华[1] 豆亚伟[1] 王宏涛[1] 田伟[1] 罗向晖[1] 段宝军[2] CHEN Yao-hua;DOU Ya-wei;WANG Hong-tao;TIAN Wei;LUO Xiang-hui;DUAN Bao-jun(Department of Thoracic Surgery,Shaanxi Provincial People's Hospital,Xi'an,Shaanxi,710068,China;Department of Oncology,Shaanxi Provincial People's Hospital,Xi'an,Shaanxi,710068,China)

机构地区：[1]陕西省人民医院胸外科,陕西西安710068 [2]陕西省人民医院肿瘤内科,陕西西安710068

出　　处：《现代生物医学进展》2022年第3期442-446,共5页Progress in Modern Biomedicine

基　　金：陕西省科技厅面上项目(2018JM7113)。

摘　　要：目的:探讨与研究青蒿琥酯对肺癌裸鼠新生血管生成的影响及机制。方法:27只裸小鼠随机平分为3组-肺癌组、青蒿琥酯1组与青蒿琥酯2组,每组9只。所有小鼠都给予腹腔注射肺癌A549细胞成瘤,致瘤成功后肺癌组、青蒿琥酯1组与青蒿琥酯2组组采用磷酸盐缓冲液、2.0 mg/mL与4.0 mg/mL的青蒿琥酯进行灌胃,1次/d,持续10 d。结果:所有小鼠都致瘤成功,青蒿琥酯1组、青蒿琥酯2组的移植瘤重量低于肺癌组(P<0.05),抑瘤率高于肺癌组(P<0.05),青蒿琥酯2组与青蒿琥酯1组对比差异有统计学意义(P<0.05)。青蒿琥酯2组、青蒿琥酯1组的移植瘤细胞凋亡指数高于肺癌组(P<0.05),青蒿琥酯2组高于青蒿琥酯1组(P<0.05)。青蒿琥酯2组、青蒿琥酯1组的移植瘤组织周围淋巴管密度低于肺癌组(P<0.05),青蒿琥酯2组低于青蒿琥酯1组(P<0.05)。青蒿琥酯2组、青蒿琥酯1组的血清肿瘤坏死因子(Tumor necrosis factor,TNF)-α与白介素(Interleukin,IL)-6水平低于肺癌组(P<0.05),青蒿琥酯2组低于青蒿琥酯1组(P<0.05)。青蒿琥酯2组、青蒿琥酯1组的移植瘤结缔组织生长因子(Connective tissue growth factor,CTGF)、血管内皮生长因子(Vascular endothelial growth factor,VEGF)蛋白相对表达水平低于肺癌组(P<0.05),青蒿琥酯2组低于青蒿琥酯1组(P<0.05)。结论:青蒿琥酯在肺癌裸鼠的应用能抑制CTGF、VEGF蛋白表达,降低炎症因子的表达,促进肿瘤细胞凋亡,从而发挥抑制肿瘤增殖与新生血管生成的作用。Objective: To explore and study the effect and mechanism of artesunate on angiogenesis in nude mice with lung cancer. Methods: 27 cases of nude mice were randomly divided into 3 groups-lung cancer group, artesunate 1 group and artesunate 2 groups,with 9 cases in each group. All mice were given intraperitoneal injection of lung cancer A549 cells for tumorigenesis, After successfully tumorigenesis, the lung cancer group, artesunate group 1 and artesunate group 2 were given phosphate buffer, 2.0 mg/mL and 4.0 mg/mL artesunate for intragastric administration, once a day, lasted 10 d. Results: All cases were successfully tumorigenic. The weight of transplanted tumors in artesunate 1 group and artesunate 2 group were lower than that of the lung cancer group(P<0.05), and the tumor inhibition rate were higher than that of the lung cancer group(P<0.05), there were statistically significant difference compared between artesunate group 2 and artesunate group 1(P<0.05). The apoptosis index of transplanted tumor cells in artesunate group 2 and artesunate group 1 were higher than that of lung cancer group(P<0.05), and artesunate group 2 were higher than artesunate group 1(P<0.05). The density of lymphatic vessels around transplanted tumor tissues in artesunate 2 group and artesunate 1 group were lower than that of lung cancer group(P<0.05), and artesunate 2 group were lower than artesunate 1 group(P<0.05). The levels of serum tumor necrosis factor(TNF)-α and interleukin(IL)-6 in artesunate group 2 and artesunate group 1 were lower than those in lung cancer group(P<0.05), the artesunate group 2 were lower than the artesunate 1 group(P<0.05). The relative expression levels of connective tissue growth factor(CTGF)and vascular endothelial growth factor(VEGF) proteins in transplanted tumors in artesunate group 2 and artesunate group 1 were lower than those in the lung cancer group(P<0.05), the artesunate group 2 were lower than the artesunate group 1(P<0.05). Conclusion: The application of artesunate in nude mice with lung cancer

关 键 词：青蒿琥酯 肺癌 裸鼠 新生血管生成 结缔组织生长因子 血管内皮生长因子 细胞凋亡 

分 类 号：R-33[医药卫生] R734.2