鼠巨细胞病毒感染裸鼠肝脏损伤模型的建立  

Establishment of Liver Injury Model in Nude Mice Infected with Murine Cytomegalovirus

作　　者：宋孙莉 柴竹洁 顾绍庆[2] 方圆[2] 詹纪春 SONG Sun-Li;CHAI Zhu-jie;GU Shao-qing;FANG Yuan;ZHAN Ji-chun(Medical College of Jiangsu University,Zhenjiang,Jiangsu,212013,China;Department of Pediatrics,People's Hospital Affiliated to Jiangsu University,Zhenjiang,Jiangsu,212002,China)

机构地区：[1]江苏大学医学院,江苏镇江212013 [2]江苏大学附属人民医院儿科,江苏镇江212002

出　　处：《现代生物医学进展》2022年第4期621-625,共5页Progress in Modern Biomedicine

基　　金：江苏省自然科学基金项目(BK20151334);镇江市重点研发计划社会发展项目(SH2018038)。

摘　　要：目的:建立鼠巨细胞病毒(MCMV)感染BALB/c裸鼠肝脏损伤的模型。方法:健康SPF级BALB/c裸鼠10只随机分为实验组和对照组,每组5只。实验组小鼠每只经腹腔注射接种250μL MCMV病毒悬液,对照组小鼠每只腹腔接种250μL DMEM培养液,于接种后第7天处死,无菌分离其肝脏,通过测定肝组织谷丙转氨酶(ALT)、实时荧光定量PCR检测MCMV DNA拷贝数、苏木精-伊红(HE)染色等方法,观察裸鼠肝脏组织的受损情况。结果:所有实验组的裸鼠均出现了不同程度的腹水;实验组裸鼠测定的肝组织ALT值较对照组明显上升(P<0.05);实时荧光定量PCR检测出实验组裸鼠肝脏MCMV DNA呈阳性;实验组肝脏病理切片HE染色可见大量炎症细胞浸润,肝细胞嗜酸性变,可见不规则包涵体,而对照组正常。结论:经腹腔注射250μL MCMV病毒悬液7天后成功构建了裸鼠肝脏损伤的模型,为探究人巨细胞病毒(HCMV)的发病机制以及抗病毒新药和疫苗的研发提供了有利条件。Objective: To establish a liver injury model in BALB/c nude mice infected with murine cytomegalovirus(MCMV).Methods: 10 healthy SPF BALB/c nude mice were randomly divided into experimental group and control group, each group 5. The mice in the experimental group were intraperitoneally inoculated with 250 μL MCMV virus suspension, and the mice in control group were intraperitoneally inoculated with 250 μL DMEM culture medium, the mice were sacrificed 7 days after inoculation, and their livers were aseptically separated. The liver tissue damage of nude mice was observed by measuring alanine aminotransferase(ALT) in liver tissue,real-time fluorescence quantitative PCR for detection MCMV DNA copy number and hematoxylin-eosin(HE) staining and other methods. Results: Nude mice in all the experimental groups showed ascites to varying degrees. ALT value in liver tissue of nude mice in experimental group was significantly higher than that in control group(P<0.05). Real-time fluorescence quantitative PCR detected that Liver MCMV DNA of nude mice in the experimental group was positive. HE staining of pathological sections of liver in the experimental group showed a large number of inflammatory cell infiltration, eosinophilic changes of liver cells, and irregular inclusion bodies, while the control group was normal. Conclusion: The liver injury model of nude mice is successfully established after intraperitoneal injection of 250 μL MCMV suspension for 7 days, which provided a beneficial condition for the study of the pathogenesis of human cytometomethovirus(HCMV) and the research and development of antiviral drugs and vaccines.

关 键 词：鼠巨细胞病毒 肝脏损伤 BALB/C裸鼠 模型 

分 类 号：R-33[医药卫生] R657.3