金丝桃苷对高脂饮食诱导ApoE^(-/-)小鼠代谢相关脂肪性肝病的保护作用  

Protective effect of hyperoside on non-alcoholic fatty liver disease in ApoE^(-/-) mice induced by high-fat diet

作　　者：关晓娇 赵文婷[2] 闵冬雨[3] 刘驰 尚永婧 胡丽萍[1] GUAN Xiaojiao;ZHAO Wenting;MIN Dongyu;LIU Chi;SHANG Yongjing;HU Liping(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;China Agricultural University,Beijing 100083,China;Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]中国农业大学,北京100083 [3]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《药物评价研究》2022年第2期281-286,共6页Drug Evaluation Research

基　　金：辽宁省自然科学基金面上项目(2015010708)。

摘　　要：目的探讨金丝桃苷对代谢相关脂肪性肝病(MAFLD)的保护作用。方法将36只ApoE^(-/-)小鼠,随机分为模型组、金丝桃苷(200 mg/kg)组和辛伐他汀(阳性对照药,5.2 mg/kg)组,每组12只;另将12只C57BL/6小鼠作为对照组。对照组给予普通饲料,其他3组均给予高脂饲料构建MAFLD模型,同时每天ig给药2次,对照组和模型组ig 0.5%CMC-Na溶液,连续给药12周。HE染色法观察小鼠肝脏组织病理变化;油红O染色法观察小鼠肝脏组织内脂肪沉积情况;全血项分析仪检测小鼠血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)含量;ELISA法检测小鼠肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平。结果与模型组比较,金丝桃苷可以减小模型小鼠肝脏细胞空洞和炎症浸润;显著改善脂质沉积(P<0.01);显著降低血清LDL-C、AST、ALT水平(P<0.01);显著降低肝脏MDA、IL-6和TNF-α水平(P<0.01),显著升高肝脏SOD和GSH-Px水平(P<0.05、0.01)。结论金丝桃苷通过减少脂质囤积、减轻氧化应激、抑制炎症反应发挥对MAFLD的保护作用。Objective To explore the protective effect of hyperoside on non-alcoholic fatty liver disease.Methods Thirty-six ApoE^(-/-) mice were randomly divided into model group,hyperin(200 mg/kg)group and simvastatin(positive control drug,5.2 mg/kg)group,with 12 mice in each group.Another 12 C57BL/6 mice were used as control group.The control group was given ordinary diet,and the other three groups were given high-fat diet to construct the MAFLD model,and the control group and the model group were given 0.5%CMC-Na solution intragaally twice a day,for 12 weeks.HE staining method was used to observe the pathological changes of mouse liver.Oil red O staining method to observe the fat content in mouse liver tissue.Whole blood analyzer to detect total cholesterol(TC),triglyceride(TG)and high-density lipoprotein cholesterol(HDL-C)in mouse serum,Low-density lipoprotein cholesterol(LDL-C),aspartate aminotransferase(AST)and alanine aminotransferase(ALT)content.ELISA method was used to detect malondialdehyde(MDA)and superoxide dismutase(SOD)in mouse liver tissue,glutathione peroxidase(GSH-Px),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)content.Results Hyperoside can reduce the area of cavities and inflammatory infiltration of mouse liver cells and improve the condition of lipid deposition(P<0.01).Hyperin significantly decreased the serum levels of TC,LDL-C,HDL-C,AST and ALT(P<0.01),decreased the levels of MDA,IL-6 and TNF-αin liver significantly(P<0.01),and increased the levels of SOD and GSH-Px in liver significantly(P<0.05,0.01).Conclusion Hyperin can slow down the pathological process of non-alcoholic fatty liver disease,and its protective effect may be achieved by reducing lipid accumulation,reducing oxidative stress,and inhibiting inflammation.

关 键 词：金丝桃苷 代谢相关脂肪性肝病 ApoE^(-/-)小鼠 脂质 氧化应激 炎症 

分 类 号：R285.5[医药卫生—中药学]