长链非编码RNA GAS5对乳腺癌细胞增殖、周期和凋亡的影响及其机制  

Effects of long non-coding RNA GAS5 on proliferation, cycle and apoptosis of breast cancer cells and its molecular mechanism

作　　者：姜丽娜[1] 祁川川 陈艳丽 刘薇[1] Jiang Lina;Qi Chuanchuan;Chen Yanli;Liu Wei(Department of Breast Surgery,the People’s Hospital of Zhengzhou,Zhengzhou 450003,China)

机构地区：[1]郑州人民医院乳腺外科,450003

出　　处：《中华实验外科杂志》2022年第2期261-264,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨长链非编码RNA(lncRNA) GAS5对乳腺癌细胞增殖、周期和凋亡的影响及其分子机制。方法选取郑州人民医院2018年12月到2021年6月手术切除的102例乳腺癌和癌旁组织作为研究对象, 采用荧光定量聚合酶链反应(PCR)分析lncRNA GAS5和微小RNA(miR)-106b-5p表达水平;乳腺癌MCF-7细胞系随机分为lncRNA对照组、lncRNA GAS5组。采用细胞计数试剂(CCK-8)分析两组细胞增殖能力;采用流式细胞术分析两组细胞的周期和凋亡水平;采用荧光素酶基因报告实验检测lncRNA GAS5和miR-106b-5p的关系。蛋白质印迹法(Western blot)分析增殖、周期和凋亡相关蛋白表达水平。计量资料比较采用t检验。结果乳腺癌组织lncRNA GAS5表达水平(0.41±0.11)明显低于癌旁组织lncRNA GAS5表达水平(1.20±0.18), 差异有统计学意义(t=5.901, P<0.05)。乳腺癌组织miR-106b-5p表达水平(1.09±0.16)明显高于乳腺癌组织miR-106b-5p表达水平(0.57±0.13), 差异有统计学意义(t=4.109, P<0.05)。lncRNA对照组细胞48 h吸光度值(1.87±0.20)高于lncRNA GAS5组细胞吸光度值(1.23±0.14), 差异有统计学意义(t=3.128, P<0.05)。lncRNA对照组细胞G0/G1期比例[(32.89±3.19)%]明显低于lncRNA GAS5组细胞G0/G1期比例[(47.83±5.32)%], 差异有统计学意义(t=4.328, P<0.05)。lncRNA对照组细胞S期比例[(36.82±4.84)%]明显低于lncRNA GAS5组细胞S期比例[(27.48±4.11)%], 差异有统计学意义(t=3.891, P<0.05)。lncRNA对照组细胞凋亡比例[(3.48±0.79)%]低于lncRNA GAS5细胞[(18.59±3.81)%], 差异有统计学意义(t=3.990, P<0.05)。miR-106b-5p是lncRNA GAS5的靶点。lncRNA对照组细胞miR-106b-5p表达水平(1.15±0.17)明显低于lncRNA GAS5组细胞miR-160b-5p表达水平(0.58±0.13), 差异有统计学意义(t=4.012, P<0.05)。结论 lncRNA GAS5在乳腺癌组织中表达下调, 通过与miR-106B-5p吸附结合, 调节乳腺癌细胞增殖、周期和凋亡等生物学过程。Objective To investigate the effect and molecular mechanism of long non-coding RNA(lncRNA)GAS5 on proliferation,cycle and apoptosis of breast cancer cells.Methods Totally,102 cases of breast cancer and adjacent tissues were selected from December 2018 to June 2021 in our hospital as objects of study.The expression levels of lncRNA GAS5 and microRNA(miR)-106b-5p were analyzed using fluorescence quantitative polymerase chain reaction(PCR).MCF-7 cell lines of breast cancer were randomly divided into lncRNA control group and lncRNA GAS5 group.The cell proliferation ability of the two groups was analyzed by cell counting reagent(CCK-8).Cell cycle and apoptosis of the two groups were analyzed by flow cytometry.The relationship between lncRNA GAS5 and miR-106b-5p was verified by Luciferase gene reporter assay.The expression levels of miR-106b-5p were analyzed by real time PCR.The measurement data were compared by t-test.Results The expression level of lncRNA GAS5 in breast cancer tissues(0.41±0.11)was significantly lower than that in adjacent tissue(1.20±0.18,t=5.901,P<0.05).The expression level of miR-106b-5p in breast cancer tissues(1.09±0.16)was significantly higher than that in breast cancer tissues(0.57±0.13,t=4.109,P<0.05).The 48-h light absorption value of cells in lncRNA control group(1.87±0.20)was significantly higher than that in lncRNA GAS5 group(1.23±0.14,t=3.128,P<0.05).The ratio of cells in G0/G1 phase in lncRNA control group[(32.89±3.19)%]was significantly lower than that in lncRNA GAS5 group[(47.83±5.32)%,t=4.328,P<0.05].The proportion of cells in S phase in lncRNA control group[(36.82±4.84)%]was significantly lower than that in lncRNA GAS5 group[(27.48±4.11)%,t=3.891,P<0.05].The apoptosis rate of lncRNA control group[(3.48±0.79)%]was lower than that of lncRNA GAS5 group[(18.59±3.81)%,t=3.990,P<0.05].MiR-106b-5p was the target of lncRNA GAS5.The expression level of miR-106b-5p in lncRNA control group(1.15±0.17)was significantly lower than that in lncRNA GAS5 group(0.58±0.13,t=4.012,P<0.05).

关 键 词：长链非编码RNA 微小RNA 乳腺癌 增殖 凋亡 周期 

分 类 号：R737.9[医药卫生—肿瘤]