龋病儿童唾液菌群动态变化及功能分析  

Dynamic Microbial Shifts and Functional Analysis of Saliva Microbial Communities with Caries Children

作　　者：王媛 张杰[1] 凌宗欣[2] 邓淑丽[1] WANG Yuan;ZHANG Jie;LING Zong-xin;DENG Shu-li(Stomatology Hospital,School of Stomatology,Zhejiang University School of Medicine,Clinical Research Center for Oral Diseases of Zhejiang Province,Key Laboratory of Oral Biomedical Research of Zhejiang Province,and Cancer Center of Zhejiang University,Hangzhou 310016,China;State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,the First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,China)

机构地区：[1]浙江大学医学院附属口腔医院·浙江大学口腔医学院·浙江省口腔疾病临床医学研究中心·浙江省口腔生物医学研究重点实验室·浙江大学癌症研究院,杭州310016 [2]传染病诊治国家重点实验室,浙江大学医学院附属第一医院,杭州310003

出　　处：《四川大学学报（医学版）》2022年第2期242-249,共8页Journal of Sichuan University(Medical Sciences)

基　　金：浙江省“尖兵”“领雁”研发攻关计划(No.2022C03060);国家自然科学基金项目(No.81371142);浙江省自然科学基金项目(No.LQ19H140002);中国医学科学院中央级公益性科研院所基本科研业务费专项资金(No.2020-JKCS-025)资助。

摘　　要：目的观察龋病和无龋儿童唾液菌群的动态变化,同时对二者口腔微生态在糖代谢及多种氨基酸合成转运过程中的功能差异进行分析。方法采用宏基因组学的方法分别对10例无龋儿童和10例龋病儿童唾液菌群进行组成及功能分析,6个月后,进一步利用PacBio SMRT测序技术,分析两组儿童口腔菌群变化,探索疾病和健康状态下,口腔菌群变化规律,从组成到功能对儿童口腔微生态进行全面解读。结果随时间推移,两组儿童口腔微生态组成发生显著改变,在门水平上,菌群变化趋势一致,Firmicutes比例增加,而Actinobacteria和Bacteroidetes比例降低。在属水平上,两组儿童唾液菌群的变化出现差异,其中,Lactobacillus、Methylobacterium和Megasphaera在龋病组儿童中的丰度呈上升趋势,而在无龋组儿童中呈下降趋势。在种水平上,隶属于乳杆菌属的L.fermentum、L.gasseri、L.oris、S.downei等菌株在龋病儿童中呈上升趋势,但在无龋儿童中始终处于较低水平。S.gordonii以及S.mutans在龋病儿童中有一定程度下降,但在无龋儿童中始终处于低水平。S.mutans和C.gracilis等菌种与龋失补牙数(decayed,missing and filled teeth,dmft)呈正相关,而N.flavescens与dmft呈负相关。三羧酸循环关键节点相关基因,gltA、icd以及mqo,谷氨酸生成相关基因gudB以及精氨酸生成相关基因argAB/C/J在无龋儿童中丰度显著增加。同时,电子传递链中的NADH脱氢酶相关基因nuoB/C/D/E/H/I/J/K/L/M在无龋儿童中的丰度显著增加。结论儿童唾液菌群是动态变化的,其变化趋势与口腔健康状态有关,健康的口腔微生态中有更广泛的氧化磷酸化,谷氨酸以及精氨酸等氨基酸生成和转运也更为活跃。Objective To observe the dynamic changes in the salivary microbiota of children with dental caries and those who were caries-free and to analyze the functional differences in the oral microecology of the two groups during the course of sugar metabolism and the synthesis and transport of multiple amino acids.Methods Ten children with dental caries and 10 caries-free children were enrolled.We employed Illumina metagenomics technology to analyze the composition and function of salivary microbiome in children with and without caries.Six months later,PacBio singlemolecule long-read sequencing technology was used to analyze the changes over time in the oral microbial communities of the two groups.We studied the patterns of change in the oral microbial communities under diseased or healthy conditions and attempted to offer a comprehensive interpretation of children’s oral microbiota in terms of its composition and functions.Results The composition of the oral microbiota of children with or without dental caries changed significantly over time.At the phylum level,changing trends in the salivary microbial communities of children with dental caries were in line with those in caries-free children.In these microbial communities,increased proportions of Firmicutes and decreased proportions of Actinobacteria and Bacteroidetes were found in the two groups.At the genus level,however,the two groups showed significantly different changes of the salivary microbial communities.Upward trends in the abundance of Lactobacillus,Methylobacterium,and Megasphaera were found in the caries group,while the abundance of these genera in the caries-free group showed downward trends.At the species level,L.fermentum,L.gasseri,L.oris,S.downei,and some other species belonging to the genus Lactobacillus showed upward trends in the saliva of children with caries,while they consistently stayed at a relative low level of abundance in caries-free children.The abundance of S.gordonii and S.mutans decreased to a certain extent in children with dental car

关 键 词：龋病 唾液菌群 高通量测序 氨基酸生成 

分 类 号：R788.1[医药卫生—口腔医学]