错[牙合]畸形不同矫治器患者菌斑微生物多样性及群落分析  

Microbial Diversity and Community Analysis of Dental Plaques in Orthodontic Patients Wearing Invisible Appliances and Fixed Appliances

作　　者：谢思洪 蒋倩 陈虹 郑蕾蕾 杨德琴[1,2,3] XIE Si-hong;JIANG Qian;CHEN Hong;ZHENG Lei-lei;YANG Deqin(Department of Conservative and Endodontic Dentistry,North Branch,Stomatological Hospital,Chongqing Medical University,Chongqing 401147,China;Chongqing Municipal Key Laboratory of Oral Diseases and Biomedical Sciences,Chongqing 401147,China;Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Institutions,Chongqing 401147,China;Department of Orthododontics,North Campus,Stomatological Hospital,Chongqing Medical University,Chongqing 401147,China)

机构地区：[1]重庆医科大学附属口腔医院北部院区牙体牙髓科,重庆401147 [2]口腔疾病与生物医学重庆市重点实验室,重庆401147 [3]重庆市高校市级口腔生物医学工程重点实验,重庆401147 [4]重庆医科大学附属口腔医院北部院区正畸科,重庆401147

出　　处：《四川大学学报（医学版）》2022年第2期250-255,共6页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金项目(No.31970783);重庆市医学重点学科建设项目(2011年55号)资助。

摘　　要：目的比较无托槽隐形矫治和传统托槽固定矫治患者菌斑的菌群结构及其差异。方法招募使用固定矫治器和隐形矫治器的正畸患者各10名,分别采集颊舌侧菌斑,利用Illumina测序平台,基于16S rDNA序列,对40个菌斑样本进行微生物多样性和群落结构分析。结果固定矫治颊侧菌斑(FB组)的物种多样性、丰富度及均匀度均高于隐形矫治颊侧(IB组)及舌侧菌斑(IL组)(P<0.05),固定矫治舌侧菌斑(FL组)表现出较大的个体差异;各组菌群结构总体相似,但一些细菌的相对丰度存在显著差异,其中IB组放线菌、罗氏菌等与龋病及牙周病相关的菌群相对丰度更高(P<0.05);FB组具有显著差异的关键菌群较为丰富,包括粪杆菌、双歧杆菌、肠杆菌、乳酸杆菌等。结论隐形矫治与固定矫治的菌斑微生物丰度、多样性和群落组成存在显著差异,且可能参与龋病、牙周病等正畸并发症的进展,无论佩戴何种矫治器,均需加强口腔卫生维护。Objective To explore the microbial diversity and community structure of dental plaques in orthodontic patients with invisible appliances and fixed appliances and to study the differences.Methods Ten orthodontic patients wearing invisible appliances(I)and ten wearing fixed appliances(F)were recruited.Dental plaques were collected from both buccal(B)and lingual(L)sides.Based on 16S rDNA,40 dental plaque samples were analyzed after Illumina sequencing.Results The microbial diversity,abundance and evenness of the FB group were significantly higher than those of the IB and IL groups(P<0.05),while the FL group showed substantial individual differences.The community structures were generally similar among the four groups,but significant differences in the relative abundance of some bacteria were found.The IB group showed higher abundances of Actinomycetes and Rosella(P<0.05),which were considered to be involved in dental caries and periodontal diseases.Some key communities showing significant differences were significantly enriched in the FB group,including Coprobacillus,Bifidobacterium,Enterobacterium,Lactobacillus,etc..Conclusion Dental plaques in patients wearing invisible appliances and fixed appliances showed significantly different microbial abundance,diversity and composition,which may be involved in orthodontic complications such as dental caries and periodontal diseases.Orthodontic patients need strengthened measures for oral hygiene maintenance,no matter what kind of appliances they wear.

关 键 词：隐形矫治 固定矫治 菌斑生物膜 微生物 

分 类 号：R783.5[医药卫生—口腔医学]