Overoxidized poly(3,4-ethylenedioxythiophene)-gold nanoparticles-graphene-modified electrode for the simultaneous detection of dopamine and uric acid in the presence of ascorbic acid  

作　　者：Junqiang Pan Mei Liu Dandan Li Haonan Zheng Dongdong Zhang 

机构地区：[1]Department of Cardiovascular Medicine,Xi'an Central Hospital,Xi'an,710061,China [2]School of Pharmacy,Xi'an Jiaotong University Health Science Center,Xi'an,710003,China

出　　处：《Journal of Pharmaceutical Analysis》2021年第6期699-708,共10页药物分析学报（英文版）

基　　金：Financial supports from the Natural Science Foundation of Shaanxi Province,China(Grant No.:2020JM-652);Fundamental Research Funds for the Central Universities of Xi’an Jiaotong University(Grant No.:xzy012020054);Cultivation Project of Xi’an Health Committee(Grant No.:2020MS02)。

摘　　要：An innovative,ternary nanocomposite composed of overoxidized poly(3,4-ethylenedioxythiophene)(OPEDOT),gold nanoparticles(Au NPs),and electrochemically reduced graphene oxide(ERGO)was prepared on a glassy carbon electrode(GCE)(OPEDOT-Au NPs-ERGO/GCE)through homogeneous chemical reactions and heterogeneous electrochemical methods.The morphology,composition,and structure of this nanocomposite were characterized by transmission electron microscopy,scanning electron microscopy,X-ray diffraction,and X-ray photoelectron spectroscopy.The electrochemical properties of the OPEDOT-Au NPs-ERGO/GCE were investigated by cyclic voltammetry using potassium ferricyanide and hexaammineruthenium(III)chloride redox probe systems.This modified electrode shows excellent electro-catalytic activity for dopamine(DA)and uric acid(UA)under physiological p H conditions,but inhibits the oxidation of ascorbic acid(AA).Linear voltammetric responses were obtained when DA concentrations of approximately 4.0-100μM and UA concentrations of approximately 20-100μM were used.The detection limits(S/N=3)for DA and UA were 1.0 and 5.0μM,respectively,under physiological conditions and in the presence of 1.0 m M of AA.This developed method was applied to the simultaneous detection of DA and UA in human urine,where satisfactory recoveries from 96.7%to 105.0%were observed.This work demonstrates that the developed OPEDOT-Au NPs-ERGO ternary nanocomposite,with its excellent ion-selectivity and electro-catalytic activity,is a promising candidate for the simultaneous detection of DA and UA in the presence of AA in physiological and pathological studies.

关 键 词：GRAPHENE Poly(3 4-ethylenedioxythiophene) OVEROXIDATION DOPAMINE Uric acid Ascorbic acid 

分 类 号：R318.08[医药卫生—生物医学工程] TB33[医药卫生—基础医学]