斑鸠霉素调节PANC-1细胞系的糖酵解过程  

Ikarugamycin regulates the glycolysis process of PANC-1 cell line

作　　者：陈辉[1] 曾陶飞 厉泽宇 王怀涛[1] 周磊[1] 谭晓冬[1] CHEN Hui;ZENG Tao-fei;LI Ze-yu;WANG Huai-tao;ZHOU Lei;TAN Xiao-dong(Department of General Surgery,Shengjing Hospital of China Medical University,Shenyang 110000,Liaoning,China)

机构地区：[1]中国医科大学附属盛京医院普通外科,沈阳110000

出　　处：《医学研究生学报》2022年第2期147-153,共7页Journal of Medical Postgraduates

基　　金：中央引导地方科技发展专项资金(2020JH6/10500055)。

摘　　要：目的 胰腺癌是恶性腹部肿瘤,以5年生存率奇低,致死率非常高为特征。文章基于生物信息学方法挖掘生物医学数据库数据,识别Ikarugamycin(斑鸠霉素)作用于胰腺导管腺癌(PDAC)的潜在靶标基因。方法 从基因表达汇编(GEO)数据库中下载数据集GSE120713,通过R软件对数据进行校正并鉴定差异表达基因(DEGs),绘制火山图和热图,并对鉴定出的DEGs进行GO富集分析和KEGG信号通路富集分析。然后,利用STRING网站和Cytoscape软件对DEGs进行蛋白质相互作用分析,借助Cytohubba插件识别出10个hub基因。最后,分别在GEPIA2和Kaplan-Meier Plotter网站上验证这些hub基因的表达及预后情况。结果 GEPIA2和Kaplan-Meier Plotter的结果提示:DDIT3、GADD45A、LOX、MMP1、SERPINE1和TXNIP同时满足在PC中表达及预后均有统计学意义,被确定为hub基因。结论 斑鸠霉素可能通过下调DDIT3或LOX的表达,参与PANC-1细胞系糖酵解过程,从而发挥对PANC-1细胞的抑制作用,该研究有助于发现斑鸠霉素治疗PDAC的潜在机制。Objective Pancreatic cancer is a malignant abdominal tumor characterized by extremely low 5-year survival rate and high mortality rate. Based on bioinformatics methods, this article mined biomedical database data to identify the potential target gene of Ikarugamycin in pancreatic ductal adenocarcinoma(PDAC). Methods First, Data set GSE120713 was downloaded from gene Expression Compilation(GEO) database. R software was used to correct data and identify differentially expressed genes(DEGs). Volcano map and heat map were drawn and GO enrichment analysis and KEGG signal pathway enrichment analysis were performed on identified DEGs. Then, DEGs protein interaction was analyzed by using the STRING website and Cytoscape software, and 10 HUB genes were identified by Cytohubba plug-in. Finally, the expression and prognosis of these hub genes were verified on GEPIA2 and Kaplan-Meier Plotter, respectively. Results Results of GEPIA2 and Kaplan-Meier Plotter suggested that DDIT3, GADD45A, LOX, MMP1, SERPINE1 and TXNIP met the same expression and prognosis in PC with statistical significance, and were identified as HUB genes. Conclusion Ikarugamycin may participate in the glycolytic process of PANC-1 cell line by down-regulating the expression of DDIT3 and LOX, thus exerting an inhibitory effect on PANC-1 cell, and this study would help to explore the potential mechanism of Ikarugamycin for the treatment of pancreatic cancer.

关 键 词：胰腺导管腺癌 斑鸠霉素 生物信息学分析 差异表达基因 

分 类 号：R735.9[医药卫生—肿瘤]