天然低共熔溶剂提取翠云草中穗花杉双黄酮工艺的优化  

Natural Deep Eutectic Solvent Extraction of Amentoflavone from Selaginella uncinata

作　　者：李刚 蒋永梅 雷杰 张凤 黎维维 李思慧 刘超 刘莉 余家奇 王刚 LI Gang;JIANG Yongmei;LEI Jie;ZHANG Feng;LI Weiwei;LI Sihui;LIU Chao;LIU Li;YU Jiaqi;WANG Gang(Zunyi Medical University,Zunyi 563000,China;The Third Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]遵义医科大学药学院,贵州遵义563000 [2]遵义医科大学第三附属医院,贵州遵义563000

出　　处：《食品工业科技》2022年第8期180-187,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金(81860697)。

摘　　要：目的:采用低共熔溶剂提取翠云草中穗花杉双黄酮(amentoflavone,AME),优化提取工艺参数。方法:合成了3种低共熔溶剂并进行筛选,然后对提取效果最佳的低共熔溶剂进行结构分析,研究提取时间、提取温度、提取功率、液固比对翠云草中AME提取量的影响,再进一步运用响应面设计技术优化提取条件,并与传统提取方法(浸渍法、渗漉法、超声法)比较。结果:筛选出适合提取AME的溶剂为氯化胆碱:4-松油醇,最佳的提取条件为:低共熔溶剂(氯化胆碱与4-松油醇摩尔比1:5)含水量30%、超声功率为280 W、液固比为16:1 mL/g、提取温度为48℃、提取时间为24 min,AME最大提取量为(0.941±0.07)mg/g。与超声法、冷浸法、热浸法比较,低共熔溶剂提取AME的含量提高了近5倍,且时间缩短了1/10(P<0.05)。结论:低共熔溶剂提取法是一种高效、快速且简便的提取方法,可为中药有效成分的提取研究提供一些参考。Objective:The deep eutectic solvent was used to extract the amentoflavone(AME)of Selaginella uncinata and the extraction process parameters were optimized.Method:Three deep eutectic solvents were synthesized and screened,and then the structure of the deep eutectic solvent with the best extraction effect was characterized.The effect of extraction time,extraction temperature,extraction power,and liquid-solid ratio on the extraction effect of amentoflavone from Selaginella uncinata were investigated.The response surface design technology was further used to optimize the extraction conditions,and compared with traditional extraction methods(dipping method,percolation method,ultrasonic method).Results:The suitable deep eutectic solvent for extracting AME was selected as choline chloride:4-terpineol.The best extraction conditions were:Ultrasonic power of 280 W,liquid-to-solid ratio of 16:1 mL/g,extraction temperature of 48℃,extraction time of 24 min,and AME maximum extraction of(0.941±0.07)mg/g.Compared with the ultrasonic method,cold soaking method,and hot soaking method,the content of AME extracted by eutectic solvent was increased nearly 5 times,and the extraction time was shortened by 1/10(P<0.05).Conclusion:The deep eutectic solvent extraction method was an efficient,fast and simple extraction method,which can provide some references for the extraction and research of the effective components of traditional Chinese medicine.

关 键 词：天然低共熔溶剂 提取 响应面法 穗花杉双黄酮 翠云草 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]