Analgesic-like activity of perillyl acetate:In vivo and in silico studies  

作　　者：Renan Braga Humberto Andrade Ryldene Cruz Mayara Maia Carolina Lima Anderson Santos AndréMiranda Allana Duarte Marcus Scotti Reinaldo Almeida Damião Sousa 

机构地区：[1]Postgraduate Program in Natural and Synthetic Bioactive Products,Federal University of Paraíba,58051-970,João Pessoa,PB,Brazil [2]Research Institute for Drugs and Medicines,Federal University of Paraíba,CP 5009,João Pessoa,58051-900,PB,Brazil [3]Department of Pharmaceutical Sciences,Federal University of Paraíba,58051-970,João Pessoa,PB,Brazil

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2022年第4期156-163,共8页亚太热带生物医学杂志（英文版）

基　　金：supported by funds from the Coordination for the Improvement of Higher Education Personnel(CAPES);National Council for Scientific and Technological Development(CNPq)。

摘　　要：Objective:To evaluate the antinociceptive activity of perillyl acetate in mice and in silico simulations.Methods:The vehicle,perillyl acetate(100,150 and/or 200 mg/kg,i.p.),diazepam(2 mg/kg,i.p.)or morphine(6 mg/kg,i.p.)was administered to mice,respectively.Rotarod test,acetic acidinduced abdominal writhing,formalin-induced nociception,hot plate test,and tail-flick test were performed.Opioid receptorsinvolvement in perillyl acetate antinociceptive effect was also investigated.Results:Perillyl acetate did not affect the motor coordination of mice.However,it reduced the number of acetic acid-induced abdominal twitches and licking times in the formalin test.There was an increase of latency time in the tail-flick test of 30 and 60minutes.Pretreatment with naloxone reversed the antinociceptive effect of perillyl acetate(200 mg/kg).In silico analysis demonstrated that perillyl acetate could bind toμ-opioid receptors.Conclusions:Perillyl acetate has antinociceptive effect at the spinal level in animal nociception models,without affecting the locomotor integrity and possibly throughμ-opioid receptors.In silico studies have suggested that perillyl acetate can act as aμ-opioid receptor agonist.

关 键 词：ANALGESIC Biological products OPIOID Essential oil Pain MONOTERPENE Perillyl acetate 

分 类 号：R965[医药卫生—药理学]