UHPLC快速测定豆浆粉中大豆异黄酮  

Determination of Soybean Isoflavones in Soymilk Powder by UHPLC

作　　者：李永利[1] 李杰[1] 姜阳[1] 吴建军[1] 薛民杰[1] LI Yong-li;LI Jie;JIANG Yang;WU Jian-jun;XUE Min-jie(Shanghai Key Laboratory of Online Testing and Control Technology,Physical and Chemical Analysis Laboratory,Shanghai Institute of Measurement and Testing Technology,Shanghai 201203,China)

机构地区：[1]上海市计量测试技术研究院理化分析室上海市在线检测与控制技术重点实验室,上海201203

出　　处：《化学试剂》2022年第4期590-593,共4页Chemical Reagents

基　　金：上海市计量测试技术研究院项目(I00RY1911)。

摘　　要：为了同时测定豆浆粉中多种大豆异黄酮成分,建立了一种豆浆粉中大豆异黄酮的超高效液相色谱分析方法。样品由80%乙醇水溶液超声提取,经碱性条件皂化反应获得大豆异黄酮测试溶液。色谱柱采用Waters Atlantis T3柱,流动相为0.1%甲酸/水溶液-乙腈,梯度洗脱,流速为0.6 mL/min,PDA检测器分析波长为260 nm。测定方法采用外标法定量,3个浓度下加标回收率为93.80%~97.99%,相对标准偏差(RSD)均小于2.5%,检出限为0.4~0.5 mg/kg。方法重现性好,适合豆浆粉基质中大豆异黄酮的快速测定。A method was developed for simultaneous determination of soybean isoflavones in soymilk powder by ultra-high performance liquid chromatography(UHPLC).The analytes in soymilk powder were extracted by ultrasound extraction with 80% ethanol water solution,and the test solution was obtained by saponification reaction under alkaline conditions.Samples were separated over a Waters Atlantis T3 column.The mobile phase was composed of 0.1% formic acid/aqueous solution and acetonitrile with flow rate of 0.6 mL/min,and PDA detection at 260 nm was applied.The recovery was 93.80%~97.99% at three levels,the relative standard deviations(RSD) were less than 2.5%,and the limit of detection was 0.4~0.5 mg/kg.The method possesses good reproducibility,and is suitable for the determination of soybean isoflavones in soymilk powder.

关 键 词：大豆异黄酮 液相色谱 豆浆粉 含量测定 

分 类 号：O657.7[理学—分析化学]