下调缺氧诱导性脂肪酸结合蛋白5(FABP5)表达抑制Huh7细胞的促血管生成能力  

Down-regulating the expression of hypoxia-induced fatty acid binding protein 5 (FABP5) inhibits tumor angiogenesis in Huh7 cells

作　　者：庞亿寒 肖衡[2] 廖锐[2] 潘龙[2] PANG Yihan;XIAO Heng;LIAO Rui;PAN Long(Urology Laboratory,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;Department of Hepatobiliary Surgery,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院泌尿外科实验室,重庆400016 [2]重庆医科大学附属第一医院肝胆外科,重庆400016

出　　处：《细胞与分子免疫学杂志》2021年第12期1092-1097,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：重庆市基础科学与前沿技术研究项目(cstc2018jcyjAX0162)。

摘　　要：目的探讨脂肪酸结合蛋白5(FABP5)在肝癌缺氧诱导的血管生成中的作用及机制。方法Huh7细胞进行常规培养及低氧培养;FABP5小干涉RNA(FABP5 siRNA)转染敲低Huh7细胞FABP5表达,反转录PCR检测FABP5 mRNA表达,Western blot法检测FABP5、血管内皮生长因子A(VEGFA)表达,ELISA检测Huh7细胞上清液VEGFA浓度;成管实验检测Huh7细胞上清液诱导人脐静脉内皮细胞(HUVEC)的成管能力。结果低氧培养后,FABP5 mRNA以及FABP5及VEGFA蛋白表达增加;采用FABP5 siRNA转染后,低氧培养Huh7细胞VEGFA表达及分泌均下降,低氧培养Huh7细胞上清液诱导HUVEC的成管能力下降。结论缺氧诱导Huh7细胞FABP5表达增加,敲低FABP5则抑制Huh7细胞VEGFA表达及HUVEC的成管能力。Objective To investigate the role and mechanism of fatty acid binding protein 5(FABP5)in hypoxia induced angiogenesis in hepatocellular carcinoma.Methods Huh7 cells were cultured under normal and anaerobic conditions;FABP5 siRNA was transfected to knock down the expression of FABP5 in Huh7 cells;the expression of FABP5 mRNA was detected by reverse transcription PCR;the expressions of FABP5 and vascular endothelial growth factor A(VEGFA)were detected by Western blot;the concentration of VEGFA in the supernatant of Huh7 cells was detected by ELISA;and the angiogenesis of human umbilical vein endothelial cells(HUVECs)induced by the supernatant of Huh7 cells was detected by tube formation assay.Results After anaerobic culture,the expressions of FABP5 mRNA and FABP5 and VEGFA proteins were increased;after FABP5 siRNA transfection,the expression and secretion of VEGFA in anaerobic cultured Huh7 cells were decreased and the angiogenesis of HUVECs induced by supernatant of anaerobic cultured Huh7 cells were decreased.Conclusion Hypoxia induces an increase in the expression of FABP5 in Huh7 cells,and knocking down FABP5 inhibits the expression of VEGFA in Huh7 cells and the angiogenesis of HUVEC.

关 键 词：脂肪酸结合蛋白5(FABP5) HUH7细胞 血管生成 细胞缺氧 

分 类 号：Q279[生物学—细胞生物学] R73-3[医药卫生—肿瘤]