内脂素-Nampt轴在卵巢上皮癌进展中的作用  

Role of visfatin-intracellular Nampt axis in the progression of epithelial ovarian cancer

作　　者：白娥[1] 王丽[1] 李海军[2] 韩苏夏[3] BAI E;WANG Li;LI Haijun;HAN Suxia(Department of Gynecology and Obstetrics,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061;Department of Thoracic Surgery,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061;Department of Radiation Oncology,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安交通大学第一附属医院妇产科,陕西西安710061 [2]西安交通大学第一附属医院胸外科,陕西西安710061 [3]西安交通大学第一附属医院肿瘤放射治疗科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2022年第2期228-233,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81703079)。

摘　　要：目的探讨内脂素-烟酰胺磷酸核糖转移酶(Nampt)轴在卵巢上皮癌进展中的作用及对患者预后的影响。方法采用免疫组化方法检测Nampt在卵巢上皮癌及正常卵巢组织内的表达,ELISA方法测定血清内脂素浓度,并分析其与临床病理特征及患者总体生存期的关系。结果卵巢上皮癌患者血清内脂素浓度显著高于卵巢良性肿瘤患者及正常对照人群,内脂素诊断卵巢上皮癌的曲线下面积(area under curve,AUC)为0.744,界值5.95 ng/mL。卵巢上皮癌患者血清内脂素与T、N、FIGO分期相关(P<0.05)且与CA125呈正相关性(rs=0.389,P=0.001)。Nampt在卵巢上皮癌组织中阳性表达率显著升高,且与FIGO分期、血清内脂素水平相关(P<0.05)。卵巢上皮肿瘤细胞Nampt蛋白表达与患者血清内脂素水平呈正相关性(rs=0.55,P<0.001)。卵巢上皮癌患者1年、3年、5年生存率分别为98.6%、74.3%、34.3%。生存分析显示,卵巢上皮癌患者生存期与T、N、FIGO分期及血清内脂素、肿瘤细胞Nampt表达有关,且FIGO分期、肿瘤细胞Nampt表达是影响患者预后的独立因素(P<0.05)。结论卵巢上皮癌患者血清内脂素、癌组织中Nampt高表达以及T、N、FIGO分期与患者总体生存期有关,且Nampt高表达、FIGO分期是独立影响因素。这提示内脂素-Nampt轴促进卵巢上皮癌进展并影响患者预后。Objective To explore the role of visfatin-nicotinamide phosphoribosyl transferase(Nampt)axis in the progression of epithelial ovarian cancer(EOC)and its effect on the patients’prognosis.Methods Immunohistochemical analysis was used to detect the expression of Nampt protein in tissues from epithelial ovarian cancer and normal ovary;ELISA was used to determine the level of visfatin in serum.Then the two were further analyzed to estimate their effects on clinicopathological characteristics and the EOC patients’overall survival.Results The mean level of serum visfatin in these EOC patients was significantly elevated compared with that of the patients with benign ovarian tumors and normal population.ROC curve analysis showed that the area under curve(AUC)of serum visfatin for diagnosis of EOC was0.744,with a cut-off value of 5.95 ng/mL.Serum visfatin of the EOC patients was related to T,N and FIGO stage(P<0.05),and was positively correlated with CA125(rs=0.389,P=0.001).The rate of Nampt positive expression in tissues from EOC was significantly increased and correlated with FIGO stage and serum visfatin(P<0.05).Nampt protein expression in EOC was positively correlated with serum visfatin level(rs=0.55,P<0.001).The 1-year,3-year and 5-year survival rate of these patients with EOC was 98.6%,74.3%and 34.3%,respectively.Survival analysis demonstrated that the overall survival of these patients was related to T,N,FIGO stage,serum visfatin and Nampt expression in EOC,and both FIGO stage and Nampt expression were independent prognostic factors(P<0.05).Conclusion The overall survival of these EOC patients was related to T,N,FIGO stage,serum visfatin and Nampt expression,and FIGO stage and Nampt expression are independent factors predicting the outcome.This highlights that visfatin-Nampt axis promotes the progression of epithelial ovarian cancer and affects the prognosis of EOC patients.

关 键 词：卵巢上皮癌 内脂素 烟酰胺磷酸核糖转移酶(Nampt) 肥胖 预后 

分 类 号：R737.3[医药卫生—肿瘤]